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目的：对聚合酶链反应（ＰＣＲ）检测首次排空尿（ＦＶＵ）诊断男性淋病的检测效果作出评估。方法：应用ＰＣＲ对２００例男性拟似淋病就诊者的ＦＶＵ进行检测，同时取尿道拭子标本行ＰＣＲ及培养，以培养阳性及ＦＶＵ与拭子ＰＣＲ均阳性作为阳性判断标准，判断各种方法的检测效果。结果：６４例培养阳性，１２例培养阴性而 ＦＶＵ与拭子 ＰＣＲ均阳性。根据判断标准，共有 ７６例阳性。拭子 ＰＣＲ ７４例真阳性，ＰＣＲ／ＦＶＵ ７３例真阳性。 ＰＣＲ／ＦＶＵ的敏感性、特异性分别是 ９６％（７３／７６）和 ９８．４％（１２２／１２４），拭子 ＰＣＲ分别是９７．４％（７４／７６）和９９．２％（１２３／１２４），拭子培养的敏感性是８４．２％（６４／７６）。ＰＣＲ／ＦＶＵ的敏感性显著高于拭子培养（ｘ~２＝４．２７，Ｐ＜０．０５）。结论：ＰＣＲ／ＦＶＵ是具有高度敏感性和特异性的非创伤性男性淋病实验室诊断方法，ＦＶＵ可代替拭子标本行ＰＣＲ诊断男性淋病。
Objective: To evaluate the detection effect of polymerase chain reaction (PCR) on the first emptying of urine (FVU) in diagnosis of male gonorrhea. Methods: PCR was used to detect FVU in 200 male patients who were suspected to be gonorrhea. PCR and culture of urethral swab specimens were also performed. Positive PCR results and positive PCR results of FVU and swabs were used as positive criteria to determine the effect of various methods Test results. Results: 64 cases were positive for culture, 12 cases were negative for culture and PCR was positive for both FVU and swab. According to the criteria, a total of 76 cases were positive. 74 were positive for swab PCR, and 73 were positive for PCR / FVU. The sensitivity and specificity of PCR / FVU were 96% (73/76) and 98.4% (122/124) respectively, and the swab PCR was 97.4% (74/76) and 99.2% (123 / 124). The sensitivity of swab culture was 84.2% (64/76). The sensitivity of PCR / FVU was significantly higher than that of swab culture (x ~ 2 = 4.27, P <0.05). Conclusion: PCR / FVU is a highly sensitive and specific method for non-invasive laboratory diagnosis of gonorrhea in men, and FVU can replace male swine samples for PCR diagnosis of male gonorrhea.