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为了探讨体感神经在受到刺激时末梢是否有ACh释放。实验用成年健康SD纯种大鼠16只,用水合氯醛麻醉,切断Tll-L。的背根,用电刺激器以5mA、0.1ms、30Hz刺激T13-L1背根的外周瑞30分钟,用ACh敏感微电极,GGF高组抗三通道微电极放大器纪录其电位变化,实验时将ACh敏感电极刺入
In order to explore whether the body sensory nerve in the stimulation of ACh release. Sixteen adult healthy SD SD rats were anesthetized with chloral hydrate, and Tll-L was cut off. The dorsal root of T13-L1 was stimulated by electrical stimulator at 5mA, 0.1ms and 30Hz for 30 minutes. The ACh-sensitive microelectrode and GGF high-impedance tri-channel microelectrode amplifier were used to record the change of potential. Pierce the ACh sensitive electrode