论文部分内容阅读
目的探讨母体低水平铅暴露对子代大鼠免疫器官发育的影响。方法孕鼠随机分为4组,自怀孕第1天起,3个处理组分别给予醋酸铅含量为125、250、500 mg/L的饮水,对照组给予不含铅的蒸馏水。于仔鼠出生后60 d取材,检测胸腺、脾脏器系数;石墨炉原子吸收检测血清、胸腺和脾脏中铅含量;流式细胞术检测胸腺、脾脏细胞凋亡率。结果仔鼠出生后60d,血清、胸腺和脾脏内的铅含量与对照组差异无统计学意义(P>0.05);但250、500 mg/L 剂量组胸腺、脾脏器系数下降(P<0.05);流式细胞结果表明,各剂量组仔鼠胸腺细胞凋亡率均显著增加(P<0.01),500 mg/L剂量组脾脏细胞凋亡率显著增加(P<0.01)。结论妊娠期低水平铅接触可以引起仔鼠胸腺和脾脏发育障碍,其机制可能与细胞过度凋亡有关。
Objective To investigate the effect of maternal low lead exposure on the development of immune organs in offspring rats. Methods Pregnant mice were randomly divided into 4 groups. From the first day of pregnancy, the three treatment groups were given drinking water with lead acetate of 125, 250 and 500 mg / L, respectively. The control group was given lead-free distilled water. The offspring of the offspring were harvested at 60 days after birth, and the thymus and spleen coefficients were detected. The contents of lead in serum, thymus and spleen were detected by graphite furnace atomic absorption spectrometry. The apoptosis rates of thymus and spleen were detected by flow cytometry. Results There was no significant difference in lead content in serum, thymus and spleen between the pups at 60 days after birth and the control group (P> 0.05). However, the coefficients of thymus and spleen in 250,500 mg / L group decreased (P <0.05) The results of flow cytometry showed that the apoptotic rate of thymocytes in each dose group was significantly increased (P <0.01), and the apoptosis rate of spleen cells in 500 mg / L dose group was significantly increased (P <0.01). Conclusions Low levels of lead exposure during pregnancy may cause developmental impairment of the thymus and spleen in the offspring. The mechanism may be related to excessive apoptosis of cells.