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目的检测烫伤大鼠创面脓毒症发生后肝脏组织中脂代谢相关基因表达水平的变化,并分析其意义。方法将60只背部30％TBSAⅢ度烫伤大鼠随机分为创面脓毒症组和对照组,每组30只。创面脓毒症组大鼠创面涂布铜绿假单胞菌菌液,并对相应指标进行检测以确定该组大鼠是否符合创面脓毒症诊断标准。对照组除创面不涂菌液外,其余操作及检测同创面脓毒症组。两组大鼠于伤后96 h断颈处死,通过基因芯片杂交方法检测两组肝组织中表达水平有显著差异的基因,并按其功能筛选出与脂代谢相关的基因。结果两组大鼠肝组织中共检出47种表达水平差异较显著的基因,其中9种基因与脂代谢相关。创面脓毒症组大鼠这9种基因中表达上调的是与脂肪酸转运和活化功能相关的多种酶基因,表达下调的是与脂肪酸在线粒体内氧化供能相关的多种酶基因。结论烫伤大鼠创面脓毒症的发生引起肝脏组织中多种脂代谢相关基因表达水平改变,加重了烫伤后的脂代谢紊乱;初步判断脂代谢障碍的发生部位为线粒体。
Objective To detect the changes of the expression of lipid metabolism related genes in the liver tissue after burn wound sepsis in rats and to analyze its significance. Methods Sixty (30) TBSA Ⅲ degree scalded rats were randomly divided into wound sepsis group (n = 30) and control group (n = 30). Wound sepsis wounds were coated with Pseudomonas aeruginosa bacterial liquid, and the corresponding indicators were tested to determine whether the group of rats meet the diagnostic criteria of wound sepsis. In addition to the control group wound surface coating liquid, the other operation and detection of wound surface sepsis group. The rats in both groups were sacrificed at 96 h after injury, and the genes whose expression levels were significantly different between the two groups were detected by gene chip hybridization. The genes related to lipid metabolism were screened by their function. Results A total of 47 genes were found in the liver tissues of the two groups, of which 9 genes were related to lipid metabolism. In wound sepsis group, the expression of these 9 genes was up-regulated by a number of enzyme genes related to fatty acid transport and activation, and the down-regulation was related to the oxidative energy supply of fatty acids in mitochondria. Conclusion The occurrence of sepsis in burned rats caused changes in the expression of many lipid metabolism-related genes in liver tissue, aggravating the lipid metabolism disorder after scald, and initially identified the mitochondria as the site of lipid metabolism disorder.