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盐分胁迫严重影响植物生长和产量。为了研究木本植物对盐分的适应性,利用5’RACE技术,从刺槐中克隆得到液泡Na~+/H~+逆向转运蛋白基因RpNHX1。该基因长度为2281 bp,含有一个开放阅读框,编码535氨基酸残基组成的蛋白,该蛋白序列与大豆GmNHX1和拟南芥AtNHX1相似,氨基酸的同源性分别达85%和69%,RpNHX1属于NHX亚族的NHX-I分枝。用生物信息学的方法预测RpNHX1具有所有Na~+/H~+逆向转运蛋白共同的结构特点,即含有疏水的N末端,10个跨膜螺旋区域和一个具有调节功能的C端亲水区域,其中氨氯吡嗪咪敏感基序和CaM结合域也是保守的。在4和5的螺旋区域之间,存在有一串带负电荷氨基酸,显示出有规律的排列模式,这些模式在生物界中的Na~+/H~+转运蛋白中是保守的。结合亲疏水资料,我们认为Na~+/H~+转运通道结构可能存在于这一区域。另外,也分析了RpNHX1可能的糖基化、酰基化和磷酸化位点。从这些数据中可以看出,RpNHX1可能在细胞中起到Na~+区隔化作用。
Salt stress seriously affects plant growth and yield. In order to investigate the adaptability of woody plants salt using 5’RACE techniques, cloned vacuolar Na ~ + / H + from the acacia antiporter gene RpNHX1. The length of the gene is 2281 bp, contains an open reading frame encoding 535 amino acid residues of the protein, the protein sequence of Arabidopsis and soybean GmNHX1 AtNHX1 similar amino acid homology of 85% and 69%, respectively, belong to that RpNHX1 NHX subfamily of NHX-I branches. RpNHX1 prediction method having all bioinformatics Na ~ + / H ~ + N-terminus retrograde transport proteins common structural characteristics, i.e. containing a hydrophobic, transmembrane helix region 10 and a C-terminal hydrophilic region having a regulatory function, Aminopyrazole-sensitive motifs and CaM-binding domains are also conserved. Between the helical regions 4 and 5, there is a string of negatively charged amino acids, exhibits a regular arrangement pattern, these patterns in the biological world Na ~ + / H ~ + transporter protein is conserved. Combined with hydrophilicity and hydrophobicity data, we think that Na ~ + / H ~ + transport channel may exist in this region. In addition, possible glycosylation, acylation and phosphorylation sites of RpNHX1 were also analyzed. As can be seen from these data, RpNHX1 may function as a Na ~ + spacer in cells.