论文部分内容阅读
目的探讨海马组织中TOLL样受体4(TLR-4)和白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)及白细胞介素10(IL-10)在致大鼠发病机制中的作用。方法应用戊四氮(PTZ)腹腔注射建立大鼠致模型后,观察大鼠致过程中的行为学变化,分别于14、21、28和35 d留取大鼠海马组织,用酶联免疫吸附测定方法(ELISA)检测海马组织中TLR-4、IL-1β、TNF-α和IL-10的蛋白含量,用实时荧光定量PCR方法检测海马组织中TLR-4 mRNA的表达水平。结果随着致时间的延长,大鼠癫发作程度逐渐加重,但是致痫间30 d后癫发作程度有所减轻。随着癫发作的进展,海马组织中TLR-4、IL-1β、TNF-α和IL-10的蛋白含量逐渐升高(P<0.01),海马组织中TLR-4 mRNA表达水平明显增强(P<0.05或P<0.01),而在35d有所下降。TLR-4的蛋白含量与IL-1β、TNF-α和IL-10含量呈正相关(P<0.01)。结论 TOLL样受体4表达增强及炎症因子和抗炎因子的表达失衡在致发病过程中发挥着重要作用。
Objective To investigate the effects of Toll-like receptor 4 (TLR-4) and interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin 10 (IL- The role of pathogenesis. Methods The model rats induced by intraperitoneal injection of pentylenetetrazole (PTZ) were established. The behavioral changes of rats induced by percussion were observed. The hippocampus of rats were collected at 14, 21, 28 and 35 days respectively, The protein levels of TLR-4, IL-1β, TNF-α and IL-10 in hippocampus were detected by ELISA. The expression of TLR-4 mRNA in hippocampus was detected by real-time fluorescence quantitative PCR. Results With the extension of time, the severity of epileptic seizures in rats gradually increased, but the degree of epileptic seizures decreased after 30 days of epilepsy. With the progression of epileptic seizures, the protein levels of TLR-4, IL-1β, TNF-α and IL-10 in hippocampus gradually increased (P <0.01), and the expression of TLR-4 mRNA in hippocampus significantly increased P <0.05 or P <0.01), but decreased at 35 days. The protein level of TLR-4 was positively correlated with the levels of IL-1β, TNF-α and IL-10 (P <0.01). Conclusion The increased TOLL-like receptor 4 expression and imbalance of inflammatory cytokines and anti-inflammatory cytokines play an important role in the pathogenesis.