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目的:观察高原低氧环境下血小板膜糖蛋白(CD62P、CD63)表达的研究与海拔高度、缺氧状态、机体损伤程度的关系。方法:采用美国Beckman-Coulter公司产Epics XL流式细胞仪及配套试剂,该仪器利用单克隆免疫荧光标记技术和流式技术对样品进行检测。结果:(1)CD62P、CD63的检测值随海拔增高而升高,两两比较P<0.05,有统计学意义;(2)PLT计数随着海拔的增高而降低,两两比较P<0.05,有统计学意义;(3)凝血各项指标也是随着海拔增高而增高,两两比较P<0.05,有统计学意义。结论:由于高原长期缺氧、红细胞增多、血液流动缓慢、血小板聚集,可导致血管内皮细胞的损伤和许多细胞因子和炎症因子的释放,引起血小板活化而可能引起血栓,其变化与海拔高度、缺氧状态、机体损伤程度等有关。
AIM: To investigate the relationship between platelet membrane glycoprotein (CD62P, CD63) expression and altitude, hypoxia and injury in plateau under hypoxia. METHODS: Epics XL flow cytometer and its reagents were used from Beckman-Coulter Company in USA. The samples were detected by monoclonal immunofluorescence labeling and flow cytometry. Results: (1) The detection values of CD62P and CD63 increased with the elevation of altitude, P <0.05 was significant in any pairwise comparison. (2) The PLT count decreased with the elevation of altitude, P <0.05, With statistical significance; (3) coagulation indicators are also increased with the elevation above sea level, P <0.05 compared with each other, with statistical significance. CONCLUSIONS: Long-term hypoxia, increased erythrocytes, slow blood flow and platelet aggregation can lead to the injury of vascular endothelial cells and the release of many cytokines and inflammatory cytokines, causing platelet activation and possibly causing thrombus. The changes are associated with altitude, lack Oxygen status, the degree of damage to the body and so on.