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为探讨血清中粒细胞巨噬细胞集落刺激因子(GM-CSF)以及高迁移率族蛋白B1(HMGB1)水平与稽留流产的关系及其意义。应用酶联免疫吸附测定法(ELISA)测定48例正常孕期女性(对照组)及139例稽留流产患者(流产组)血清中GM-CSF及HMGB1的蛋白水平。并制备稽留流产病人绒毛组织的染色体,检查染色体异常情况。结果表明,对照组的GM-CSF阳性检出率为27.08%,平均浓度为(72.00±13.21)pg/ml,流产组血清GM-CSF的阳性检出率为46.76%,平均浓度为(414.98±75.80)pg/ml,流产组血清GM-CSF明显高于对照组,具有显著性差异(P<0.05);139例稽留流产患者中,绒毛染色体异常的有81例,染色体异常组与染色体正常组血清GM-CSF水平无显著性差异。对照组和流产组血清HMGB1检查结果均低于检测下限(0.39 ng/ml)。血清GM-CSF水平与稽留流产有密切关系。GM-CSF可能在妊娠期细胞因子网络中起重要的平衡作用。
To investigate the relationship between serum GM-CSF and high mobility group box 1 (HMGB1) levels and missed abortion and its significance. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of GM-CSF and HMGB1 in 48 normal pregnant women (control group) and 139 aborted patients (abortion group). And prepare the choroms of the villi tissues of the missed abortion patients and check the chromosomal abnormalities. The results showed that the positive rate of GM-CSF in control group was 27.08% and the average concentration was (72.00 ± 13.21) pg / ml. The positive rate of GM-CSF in abortion group was 46.76% and the average concentration was (414.98 ± 75.80) pg / ml, the serum GM-CSF in abortion group was significantly higher than that in control group (P <0.05). Among the 139 cases of missed abortion, there were 81 cases of chromosomal abnormalities, Serum GM-CSF levels were not significantly different. The results of serum HMGB1 test in control group and abortion group were lower than the detection limit (0.39 ng / ml). Serum GM-CSF levels and missed abortion are closely related. GM-CSF may play an important balance in the cytokine network during pregnancy.