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目的 :观察氧自由基对培养的胚胎海马神经元三磷酸腺苷酶 (ATPase)和生长抑素 (SOM)含量的影响以及人参皂甙 (Gin)的抗自由基作用。方法 :在细胞培养第 5天给以黄嘌呤 -黄嘌呤氧化酶 (X- XO)体系建立氧自由基损伤模型 ,并分别同时给以 Gin超氧化物岐化酶 (SOD)以及 Gin+SOD,采用组化及免疫组化染色观察各组ATPase及 SOM含量变化。结果 :X- XO组 ATPase及 SOM含量均低于对照组及各实验组 (P <0 .0 1) ,Gin+SOD及 ATPase及 SOM含量均高于 Gin及 SOD)组 (P <0 .0 1)。结论 :氧自由基可降低 ATP ase和 SOM含量 ,Gin、SOD可增加 ATPase和 SOM含量 ,二者联合应用优于单独应用。提示 Gin可能具有 SOD样作用或激活体内 SOD作用
OBJECTIVE: To observe the effect of oxygen free radicals on the content of ATPase and somatostatin (SOM) in cultured hippocampal neurons and the anti-free radical effect of gin. Methods: The oxygen free radical injury model was established on xanthine-xanthine oxidase (X-XO) system on day 5 of cell culture, and the effects of Gin superoxide dismutase (SOD) and Gin + SOD, The changes of ATPase and SOM in each group were observed by histochemical and immunohistochemical staining. Results: The contents of ATPase and SOM in X-XO group were lower than those in control group and experimental groups (P <0.01), Gin + SOD, ATPase and SOM contents were higher than those in Gin and SOD groups 1). CONCLUSION: Oxygen free radicals can decrease ATP ase and SOM contents. Gin and SOD can increase ATPase and SOM contents. The combination of them is better than single application. Suggesting that Gin may have SOD-like effects or activate SOD in vivo