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目的探讨用17β-雌二醇和孕酮构建小鼠生殖器疱疹动物模型的方法。方法实验前14d摘除雌性BALB/c小鼠卵巢,并经皮下注射17β-雌二醇、醋酸甲羟孕酮(长效孕酮)、孕酮或雌孕激素联合预处理,以生殖器单纯疱疹病毒(HSV-2)攻击小鼠生殖道,监测小鼠生殖周期、外阴病变和病毒滴度。结果孕酮预处理后小鼠进入动情间期并对HSV-2易感,长效孕酮可延长小鼠的动情间期至30d;雌二醇处理后小鼠进入动情期并对HSV-2不易感染;雌孕激素联合处理后小鼠进入动情期-动情间期,并对HSV-2易感染,表现明显的生殖器疱疹症状;雌孕激素剂量不影响易感性,也不影响小鼠感染程度和存活率。结论孕酮联合雌激素预处理,用低滴度HSV-2(333)感染,可以构建易感稳定的小鼠生殖器疱疹模型。
Objective To explore the method of constructing animal genital herpes model with 17β-estradiol and progesterone. Methods Female BALB / c mice were ovariectomized 14 days prior to the experiment and injected with 17β-estradiol, medroxyprogesterone acetate (progesterone acetate), progesterone or estrogen / progesterone combined with genital herpes simplex virus (HSV-2) to challenge the reproductive tract of mice to monitor the mouse reproductive cycle, vulvar lesions and viral titers. Results After pretreatment with progesterone, the mice entered the estrous interval and were susceptible to HSV-2. The long-acting progesterone prolonged the estrous interval of mice to 30 days. The estradiol-treated mice entered the estrus phase and the HSV-2 Not susceptible to infection; estrogen and progesterone combined treatment of mice into the period of estrus - estrus, and susceptible to HSV-2 infection, showing significant symptoms of genital herpes; estrogen and progesterone dose does not affect the susceptibility, but also does not affect the degree of infection in mice And survival rate. Conclusion Pretreatment with progesterone combined with estrogen can infect susceptible and stable genital herpes in mice infected with low titer HSV-2 (333).