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Objective To identify anti-tumor effects of the ethanol extract from radix of Actinidia chinensis(EERAC) and Evodiamine(Evo) on colon carcinoma SW480 cells,and investigate their regulation of CCR7 expression and the underlying mechanism in colon carcinoma.Methods Radix of Actinidia chinensis was extracted by ethanol.MTT assay and growth curve method were used to detect the inhibition of SW480 cells after being treated with EERAC and Evo.RT-PCT and Western-blot were used to detect the CCR7 expression regulated by EERAC and Evo at the level of protein and mRNA respectively in SW480 cells.Results MTT results showed that EERAC and Evo significantly inhibited the growth of SW480 cells with obvious time-and dose-dependent effect.The 50% inhibitory concentration(IC50) of EERAC was 9.14 mg/mL,while the IC50 of Evo was 2.11 μg/mL.RT-PCR results showed that the expression of CCR7 mRNA in EERAC and Evo group was 29.99% and 18.03% respectively,which was obviously lower than that(56.27%) in control group.Western-blot proved that the expression of CCR7 protein in EERAC and Evo group was 29.03% and 15.28% respectively,which was also significantly reduced when compared to that of 42.48% in control group.Conclusion Ethanol extract from radix of Actinidiae chinensis and Evodiamine could effectively inhibit proliferation and growth of SW480 cells,significantly down-regulate the functional expression of CCR7,and thereby suppress the tumor metastasis.
Objective To identify anti-tumor effects of the ethanol extract from radix of Actinidia chinensis (EERAC) and Evodiamine (Evo) on colon carcinoma SW480 cells, and investigate their regulation of CCR7 expression and the underlying mechanism in colon carcinoma. Methods Radix of Actinidia chinensis was extracted by ethanol. MTT assay and growth curve method were used to detect the inhibition of SW480 cells after being treated with EERAC and Evo. RT-PCT and Western-blot were used to detect the CCR7 expression regulated by EERAC and Evo at the level Results of MTT results showed that EERAC and Evo significantly inhibited the growth of SW480 cells with obvious time-and dose-dependent effect. The 50% inhibitory concentration (IC50) of EACAC was 9.14 mg / mL, while the IC50 of Evo was 2.11 μg / mL. RT-PCR results showed that the expression of CCR7 mRNA in EERAC and Evo group was 29.99% and 18.03% respectively, which was obviously lower than that (56.27%) in contro l group. Western-blot demonstrated that the expression of CCR7 protein in EERAC and Evo group was 29.03% and 15.28% respectively, which was also significantly reduced when compared to 42.48% in control group. Confcision Ethanol extract from radix of Actinidia chinensis and Evodiamine could substantially inhibit proliferation and growth of SW480 cells, significantly down-regulate the functional expression of CCR7, and therefore suppress the tumor metastasis.