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目的:采用制备单柱配合前后处理工艺来分离得到高纯度异甘草素。方法:采用正交设计优化前处理超声提取的条件,提取物经2次制备单柱梯度洗脱分离,浓缩后得目标产品。结果:超声提取最佳条件为:配制原料的质量浓度为30g/L,采用乙醇做提取溶剂,超声提取时间为60min,提取温度为20℃。选择适合的单柱进样浓度为100g/L,进样体积为60mL。单柱产品经浓缩得到质量分数在99%以上的异甘草素。结论:以柱色谱分离为核心,结合原料前处理和产品后处理工艺分离提纯异甘草素工艺简单易行,所得产品质量分数高。
OBJECTIVE: To isolate and obtain high purity isoliquiritigenin by preparing single column with pre and post treatment. Methods: Orthogonal design was used to optimize the condition of ultrasonic extraction before extraction. The extract was separated by two preparative single-column gradient elution and concentrated to obtain the target product. Results: The optimum conditions of ultrasonic extraction were as follows: the mass concentration of raw materials was 30g / L, ethanol was used as extraction solvent, ultrasonic extraction time was 60min, extraction temperature was 20 ℃. Select the appropriate single-column injection concentration of 100g / L, injection volume of 60mL. The single column product is concentrated to obtain isoliquiritigenin with the mass fraction above 99%. Conclusion: The separation and purification of isoliquiritigenin from raw material pretreatment and product post-treatment process are simple and easy with column chromatography separation as the core. The obtained product has high quality fraction.