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目的探讨YAP蛋白在临床不同分期、恶性程度不同的胃癌组织中的表达差异,及其同胃癌死亡率的影响。方法选择2013年8月至2015年1月湖北省江汉大学附属医院胃癌临床Ⅰ期组织块17例、Ⅱ期胃癌组织35例、Ⅲ期胃癌组织42例和Ⅳ期31例,以及相应的癌旁组织作为对照,采用免疫组化(IHC)和Western blot方法分别检测YAP蛋白表达水平,分析其与胃癌死亡率的相关性。结果胃癌组织中YAP表达水平显著高于癌旁组织(P=0.002 1),差异有统计学意义(P<0.05)。YAP在不同分期的胃癌病人中表达也具有差异性,且恶性程度越高的胃癌组织中,YAP表达量越多;YAP表达水平与临床Ⅲ期(P=0.020)和临床Ⅳ期(P=0.007)患者死亡率有显著性相关,YAP高表达的胃癌病人生存率显著低于YAP低表达的胃癌病人。结论胃癌的恶性程度与YAP的表达呈正相关,异常高表达YAP的胃癌病人一般伴随不良预后和高死亡率。
Objective To investigate the expression of YAP protein in gastric cancer with different clinical stages and degrees of malignancy, and its effect on gastric cancer mortality. Methods From August 2013 to January 2015, 17 cases of stage Ⅰ gastric cancer tissue, 35 cases of stage Ⅱ gastric cancer, 42 cases of stage Ⅲ gastric cancer and 31 cases of stage Ⅳ gastric cancer were included in the Affiliated Hospital of Jianghan University, Hubei Province, Tissue as a control, immunohistochemistry (IHC) and Western blot were used to detect the level of YAP protein expression, and analyze its correlation with gastric cancer mortality. Results The expression level of YAP in gastric cancer tissues was significantly higher than that in adjacent non-cancerous tissues (P = 0.002 1). The difference was statistically significant (P <0.05). YAP expression in gastric cancer patients with different stages also have differences, and the higher the degree of malignancy in gastric cancer tissues, YAP expression more; YAP expression levels and clinical stage Ⅲ (P = 0.020) and clinical stage Ⅳ (P = 0.007 ) Patient mortality was significantly correlated, YAP high expression of gastric cancer patients survival was significantly lower than YAP low expression of gastric cancer patients. Conclusion The malignant degree of gastric cancer is positively correlated with the expression of YAP. Patients with abnormally high expression of YAP in gastric cancer generally have poor prognosis and high mortality.