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以黄瓜子叶离体培养为实验系统 ,研究了 Cu2 + 对离体子叶外植体花芽分化的影响 .育苗期的培养基中缺乏 Cu2 + 显著地降低离体子叶外植体花芽、营养芽和间接花的分化率 ;离体子叶外植体培养在含有不同浓度的 Cu SO4( 0μmol/L～ 0 .6μmol/L)培养基中 ,外植体花芽和营养芽的分化率随 Cu2 + 浓度升高 ,其中以0 .4 μmol/L为最佳 ;离体子叶外植体在培养的不同时期给予 Cu2 +脉冲处理 2 4 h,发现第 5天给予 0 .4 μmol/L脉冲处理显著地促进花芽的分化 .
Cucumber cotyledon explants were cultured in vitro to study the effects of Cu2 + on flower bud differentiation in vitro cotyledon explants.The lack of Cu2 + in the culture medium at seedling stage significantly reduced flower buds, vegetative shoots and shoots of explant cotyledons The differentiation rate of flower buds and vegetative buds of explants increased with the concentration of Cu2 + in culture medium containing different concentrations of CuSO4 (0μmol / L ~ 0.6μmol / L) , Of which 0. 4 μmol / L was the best. Explant cotyledon explants were treated with Cu2 + for 24 h at different stages of culture, and it was found that pulsed with 0.4 μmol / L on day 5 significantly promoted flower buds Differentiation.