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纯化SEA在 10 -12 ～ 10 -5g/ml浓度范围对体外培养的BALB/c鼠脾细胞表现了细胞增殖诱导能力 ,并呈剂量依赖关系 ,其中 10 -7～ 10 -5g/mlSEA的作用强于最适量 ( 2 5 μg/ml)PHA。在E/T为 5∶1～ 2 0∶1条件下 ,10 -5g/mlSEA活化 48h的BALB/c鼠脾细胞对YAC 1细胞的杀伤活性高于NK细胞 ,但SEA未能增强BALB/c鼠脾细胞对B16细胞的杀伤活性。 5 μg和5 0 μgSEA体内用药对L615白血病无治疗作用 ,但转输经SEA活化的同系小鼠脾细胞对L615白血病有一定疗效。实验结果提示 ,SEA活化淋巴细胞对NK敏感的淋巴瘤、白血病有杀伤及治疗作用 ,这种作用依赖于较大数量级SEA活化淋巴细胞的存在 ,并且与SEA活化T细胞分泌的细胞因子有关
Purified SEA was able to induce cell proliferation in BALB/c mouse spleen cells cultured in a concentration range of 10 -12 to 10 -5 g/ml in a dose-dependent manner. The effect of 10 -7 - 10 -5 g/ml SEA was strong. In the optimum amount (25 μg/ml) PHA. Under the condition of E/T ratio of 5:1 to 20:1, the killing activity of BALB/c mouse spleen cells with 10-5g/ml SEA activation for 48 h was higher than that of NK cells, but SEA failed to enhance BALB/c. Murine spleen cell killing activity against B16 cells. Treatment with 5 μg and 50 μg SEA in vivo had no therapeutic effect on L615 leukemia, but transfusion of SE-activated syngeneic mouse spleen cells had a certain effect on L615 leukemia. The experimental results suggest that SEA activated lymphocytes have killing and therapeutic effects on NK-sensitive lymphomas and leukemias. This effect is dependent on the presence of large-scale SEA activated lymphocytes and is related to cytokines secreted by SEA activated T cells.