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以榅桲组培苗为实验材料,采用不同质量分数的NaCl和Na2SO4处理后,观察盐胁迫对其生理生化指标的影响.结果表明:榅桲组培苗萌芽率、增殖倍数和平均株高均随2种盐质量分数的增大而降低,并在2种盐质量分数皆为1.0%时不再萌芽,平均株高降至试验最低值,而在NaCl和Na2SO4质量分数分别为0.8%和1.0%时不再增殖;榅桲组培苗叶片膜透性随着2种盐质量分数的增大而升高且与对照差异显著,并于盐质量分数为1.0%时均达到试验最大值,而当NaCl和Na2SO4质量分数分别为0.8%和1.0%时,组培苗致死率超过50%;组培苗叶片的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均随着2种盐质量分数的增大呈先上升后下降趋势,而过氧化物酶(POD)活性则随着2种盐质量分数的增加而持续增强.研究发现,盐胁迫榅桲组培苗的相关指标均与相应对照有显著差异,其中的叶片膜透性及其SOD、CAT以及POD均可作为鉴定榅桲耐盐性评价指标.
The effects of salt stress on its physiological and biochemical indexes were observed after the tissue culture seedlings were used as experimental material and treated with NaCl and Na2SO4 with different mass fraction.The results showed that the germination rate, multiplication ratio and average plant height Decreased with the increase of the two salt contents, and no longer germinated when the two salt contents were all 1.0%. The average height of the plants decreased to the lowest value of the test. However, when the NaCl and Na2SO4 contents were 0.8% and 1.0% % Did not proliferate. The membrane permeability of Triticeae in tissue culture seedlings increased with the increase of two kinds of salt content and significant difference with the control, and reached the maximum when the salt content was 1.0% When the NaCl and Na2SO4 concentrations were 0.8% and 1.0% respectively, the lethality of tissue culture seedlings was over 50%. The activities of superoxide dismutase (SOD) and catalase (CAT) The salt content increased first and then decreased, while the peroxidase (POD) activity continued to increase with the increase of two salt contents. The results showed that the relative indexes There were significant differences with the corresponding control, in which the leaf membrane permeability and SOD, CAT and POD can be used as Given salt tolerance evaluation quince.