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目的:探讨加味茵陈蒿汤对湿热哮喘大鼠气道重塑的影响及其可能的机制。方法:50只健康雄性SD大鼠随机均分为正常组、湿热哮喘模型组、加味茵陈蒿汤中、高剂量组、地塞米松组(n=10)。采用多因素干预建立湿热哮喘大鼠模型。HE染色观察大鼠肺组织形态,ELISA法测定各组大鼠血清白介素-6(IL-6)水平,免疫组织化学染色观察大鼠气道信号转导因子-3(STAT3)和胸腺活化调节趋化因子(TARC)表达水平。采用Image-Pro Plus 6.0测定支气管总管壁厚度(Wat/Pbm)及气道平滑肌厚度(Wam/Pbm)。结果:模型组大鼠湿热哮喘表现明显,HE染色出现典型气道重塑改变。气道TARC表达和Wat/Pbm、Wam/Pbm与血清IL-6和气道STAT3表达呈正相关(P<0.05)。与模型组比较,中药中剂量组大鼠气道IL-6/STAT3表达、气道TARC表达(P<0.05)和气道Wat/Pbm(P<0.05)、Wam/Pbm(P<0.05)值明显降低。结论:加味茵陈蒿汤中剂量早期干预能显著减轻湿热哮喘大鼠气道重塑,机制可能与抑制大鼠肺组织IL-6/STAT3通路的表达有关。
Objective: To investigate the effects of Yinchenhao decoction on airway remodeling in asthmatic rats with damp heat and its possible mechanism. Methods: Fifty healthy male Sprague-Dawley rats were randomly divided into normal group, hot and humid asthma model group, plus Yinchenhao Decoction, high dose group and dexamethasone group (n = 10). Multivariate intervention was used to establish a rat model of damp-heat asthma. The morphology of rat lung was observed by HE staining. The level of interleukin-6 (IL-6) was measured by enzyme-linked immunosorbent assay (ELISA) and the expression of STAT3 and thymus Factor (TARC) expression levels. Bronchial wall thickness (Wat / Pbm) and airway smooth muscle thickness (Wam / Pbm) were measured using Image-Pro Plus 6.0. Results: The rats in the model group showed obvious damp-heat asthma, and the typical airway remodeling was observed by HE staining. Airway TARC expression and Wat / Pbm, Wam / Pbm were positively correlated with serum IL-6 and airway STAT3 expression (P <0.05). Compared with the model group, the expression of IL-6 / STAT3, TARC and airway Wat / Pbm (P <0.05) and Wam / Pbm (P <0.05) reduce. Conclusion: The early intervention with modified Yinchenhao decoction can significantly reduce the airway remodeling in rats with damp-heat asthma, and the mechanism may be related to the inhibition of IL-6 / STAT3 pathway in rat lung tissue.