论文部分内容阅读
为探讨黑质抗癫痫作用的神经化学机制，实验用大鼠４０只，１２０万Ｕ青霉素（ｉｐ）诱发其癫痫（ＥＥＧ）发作．高波幅尖波连续发放型癫痫放电稳定时：（１）电刺激黑质（１０Ｈｚ、６Ｖ、０．２ｍｓ）即刻出现癫痫放电频率下降（Ｐ＜０．０５），连续刺激２５ｍｉｎ，效应最明显时停止电刺激，癫痫放电频率随即恢复，（２）黑质内微量注射多巴胺（ＤＡ）受体激动剂盐酸阿朴吗啡５μｇ，出现癫痫放电频率抑制现象（Ｐ＜０．０５），持续６０ｍｉｎ以上，部分动作癫痫放电消失后不再复现．（３）黑质内微量注射γ氨基丁酸（ＧＡＢＡ）４～５μｇ，明显易化大鼠癫痫放电频率（Ｐ＜０．０１），４０ｍｉｎ后癫痫放电频率大约是用药前的５倍，该效应可以被黑质内微量注射印防己毒素５μｇ阻断．结果提示：激活黑质ＤＡ系统功能活动有利于对抗青霉素致大鼠癫痫发作．
In order to investigate the neurochemical mechanism of antiepileptic effect in substantia nigra, 40 epileptic rats (EEG) were induced by 40 rats and 1.2 million penicillin (ip). (1) Electrical stimulation of the substantia nigra (10Hz, 6V, 0.2ms) immediately decreased the frequency of epileptic discharge (P <0.05), continuous stimulation of 25min, the effect of the most obvious (2) Apomorphine hydrochloride, a dopamine (DA) receptor agonist, was injected into the substantia nigra at a dose of 5μg, and the frequency of epileptic discharge was inhibited (P <0.05) for more than 60min, Part of the action epileptic discharge disappeared no longer recurrence. (3) Microinjection of 4 ~ 5μg γ-aminobutyric acid (GABA) into the substantia nigra significantly attenuates the frequency of epileptic discharge in rats (P <0.01). After 40 minutes, the frequency of epileptic discharge is about 5 times higher than that of the control It can be blocked by intradermal microinjection of picrotoxin 5μg. The results suggest that: activation of substantia nigra DA system functional activity is conducive to penicillin-induced seizures in rats.