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将具有聚集诱导荧光的4-(2-吡啶乙烯基)-1,8-萘酰亚胺单元通过酰胺化反应连接到聚酰胺-胺(PAMAM)树形分子上,制备了一种水溶性吡啶萘酰亚胺-聚酰胺-胺荧光树形分子PN-PAMAM.结构经核磁共振氢谱、核磁共振碳谱、红外及高分辨质谱表征.吡啶萘酰亚胺-聚酰胺-胺树形分子PN-PAMAM具有明显的聚集诱导荧光增强(AIEE)特性.在固体态时的最大荧光发射波长为532 nm,紫外灯下发出黄绿色荧光.在纯水中的最大荧光发射波长为494 nm,荧光量子产率为4.42%.在水含量为60%的水/四氢呋喃混合溶液中,其荧光强度达到最大,荧光发射波长为485 nm,荧光量子产率增大到17.54%.制备了一种负载聚集诱导荧光染料PN-PAMAM的二氧化硅纳米粒子PN-PAMAM/Si O_2,测得该纳米粒子的荧光发射波长为473 nm,粒径约为40 nm.测定了水溶液中树形分子PN-PAMAM与乳腺癌细胞MCF-7共同孵化后的共聚焦荧光成像,得到清晰的蓝场荧光照片.研究表明,吡啶萘酰亚胺-聚酰胺-胺树形分子PN-PAMAM是一种水溶性荧光分子,具有明显的聚集诱导荧光增强特性,可广泛应用于肿瘤定位、生物追踪及纳米材料等重要领域.
A 4- (2-pyridinylvinyl) -1,8-naphthalimide unit with aggregation-induced fluorescence was attached to a polyamide-amine (PAMAM) dendrimer by an amidation reaction to prepare a water-soluble pyridine The structure of naphthalimide-polyamide-amine fluorescent dendrimer PN-PAMAM was characterized by 1H nuclear magnetic resonance, nuclear magnetic resonance carbon, infrared and high resolution mass spectrometry. Pyridine naphthalimide-polyamide-amine dendrimer PN -PAMAM has obvious aggregation-induced fluorescence enhancement (AIEE) characteristics.The maximum fluorescence emission wavelength is 532 nm in solid state, yellow-green fluorescence under UV light.The maximum fluorescence emission wavelength in pure water is 494 nm, fluorescence quantum The yield was 4.42%, and the fluorescence intensity reached the maximum at the fluorescence wavelength of 485 nm and the fluorescence quantum yield increased to 17.54% in the water / tetrahydrofuran mixed solution with the water content of 60% .A load aggregation induction Fluorescent dye PN-PAMAM silica nanoparticles PN-PAMAM / Si O_2, measured the fluorescence emission wavelength of the nanoparticle 473 nm, particle size of about 40 nm. Determination of the aqueous solution of the tree-shaped molecule PN-PAMAM and breast Confocal fluorescence imaging after co-incubation of cancer cells with MCF-7 was clear Field fluorescence photos.The research shows that the pyridine-naphthalimide-polyamide-amine dendrimer PN-PAMAM is a kind of water-soluble fluorescent molecule with obvious aggregation-induced fluorescence enhancement and can be widely used in tumor localization, biological tracking and Nano-materials and other important areas.