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研究肿瘤坏死因子α(Tumor necrosis factor-alpha,TNF-α)、粒细胞集落刺激因子(Granulocyte-colony stimulationfactor,G-CSF)对经冠状动脉灌注骨髓间充质干细胞(Mesenchymal stem cells,MSCs)移植率的影响。选用2～3月龄健康日本大耳白兔,耳缘静脉注射阿霉素制作心肌损伤模型后随机分为3组:(1)空白对照组;(2)TNF-α组;(3)G-CSF组。每组7只。空白对照组移植前种子细胞及动物均不用细胞因子干预;TNF-α组移植前1d经耳缘静脉注入TNF-α(5μg/kg),种子细胞在移植12h前加入TNF-α(10ng/ml)进行预处理;G-CSF组移植前3d开始肌肉注射G-CSF(10μg/kg),每d一次;种子细胞在移植前12h加入G-CSF(10ng/ml)进行预处理。密度梯度离心法获取MSCs,体外扩增后4,6-二脒-2-苯基吲哚(DAPI)标记,主动脉根部球囊封堵方法移植细胞;4周后制作心肌组织切片计数移植细胞并进行统计学分析。TNF-α组及G-CSF组兔心肌内移植的细胞数明显多于空白对照组,差异有统计学意义(P<0.001)。提示体外培养的MSCs和心肌损伤模型经细胞因子TNF-α、G-CSF干预后,能提高经冠状动脉移植骨髓间充质干细胞的移植率。其可能机制与细胞因子TNF-α、G-CSF能提高MSCs与心脏微血管内皮细胞(Cardiac microvasculature endothelium CMVE)的黏附能力有关。
To study the effects of tumor necrosis factor-alpha (TNF-α) and granulocyte colony stimulation factor (G-CSF) on the transplantion of mesenchymal stem cells (MSCs) Rate of impact. The rabbits were randomly divided into 3 groups: (1) blank control group; (2) TNF-α group; (3) G -CSF group. 7 per group TNF-α (5μg / kg) was infused into the TNF-α group 1 day before transplantation, and the seed cells were treated with TNF-α (10ng / ml) ). G-CSF (10μg / kg) was intramuscularly injected once a day before G-CSF administration. Seed cells were pretreated with G-CSF (10ng / ml) 12h before transplantation. MSCs were obtained by density gradient centrifugation and labeled with 4,6-diamidino-2-phenylindole (DAPI) and aortic root balloon occlusion method in vitro. After 4 weeks, And statistical analysis. The number of cells transplanted in myocardium of TNF-α group and G-CSF group was significantly more than that of the blank control group, the difference was statistically significant (P <0.001). These results suggest that MSCs transplantation and myocardial injury model can improve the transplanted rate of transplanted bone marrow mesenchymal stem cells after the intervention of cytokines TNF-α and G-CSF. The possible mechanism is related to the ability of cytokines TNF-α and G-CSF to enhance the adhesion of MSCs to Cardiac microvasculature endothelium (CMVE).