大鼠AAF肝癌琥珀酸脱氢酶定量组织化学的研究

• 作 者: 强家模 姚(?)
• 机 构: 中国科学院实验生物研究所,
• 出 处: 实验生物学报
• 发表日期: 1964年04期
• 关键词: 琥珀酸脱氢酶 AAF 大鼠 酶活力 移植过程 奶油黄 干重比 光谱吸收 反应产物 dehydrogenase

应用琥珀酸脱氢酶组织化学反应产物的定量抽提和光谱吸收分析,并以切片干重作为针算酶活力的单位,比较了大鼠正常肝、AAF所诱发的原发性肝癌及移植型肝癌中该酶活力的差异。实验结果证明当正常肝转变为肝癌时酶活力显著降低,而且在长期移植过程中继续有所减少。酶活力的降低是和粒线体数量的减少相平行的。

Quantitative extraction and spectral absorption analysis of succinate dehydrogenase histochemical reaction products were performed. The dry weight of the slices was used as the unit of the enzyme activity of the needles to compare the normal liver and AAF-induced primary liver cancer and the transplanted type of rats. The difference in the activity of this enzyme in liver cancer. The experimental results show that when the normal liver is transformed into liver cancer, the enzyme activity is significantly reduced, and it continues to decrease during long-term transplantation. The decrease in enzyme activity is parallel to the decrease in the number of mitochondria.