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【目的】粉虱种类繁多,个体微小,其种类识别与鉴定常需借助分子生物学技术。本研究旨在明确线粒体COI基因(mitochondrial cytochrome c oxidase subunit I gene)5’端和3’端序列对常见种类粉虱识别鉴定的可行性。【方法】以我国田间常见的16种粉虱为对象,以COI基因5’端(641 bp)和3’端(738 bp)序列为靶标进行比对分析,以MEGA 5.10软件的K2-P模型计算种内与种间遗传距离,以邻接法(NJ法)构建进化树并进行系统发育分析。【结果】当以5’端为靶标时,16种粉虱的种内平均遗传距离为0.0015,种间平均遗传距离为0.2897,种间遗传距离为种内遗传距离的193.1倍;而且种内、种间遗传距离没有重叠区域。当以3’端为靶标时种内平均遗传距离为0.0007,种间平均遗传距离为0.2817,种间遗传距离为种内遗传距离的402.4倍;但桑粉虱Pealius mori与烟粉虱Bemisia tabaci Asia II 1的种内和种间遗传距离重叠。系统发育分析结果显示,以5’端为靶标时,16种粉虱可以形成独立的进化分支;以3’端为靶标时,除桑粉虱与传统分类学不一致外,其余种类均可形成独立的分支。【结论】结果表明,5’端序列更适用于基于DNA条形码技术的物种识别鉴定研究。
【Purpose】 There are many kinds of whitefly and individuals are very small. Its identification and identification often require molecular biology techniques. The purpose of this study was to determine the feasibility of identification of common whitefly species at the 5 ’and 3’ end of the mitochondrial cytochrome c oxidase subunit I gene. 【Method】 A total of 16 whitefly species commonly found in the field of China were screened for the COI gene 5 ’(641 bp) and 3’ (738 bp) sequences as targets. The K-P model of MEGA 5.10 software The intraspecific and interspecific genetic distances were calculated, and the phylogenetic tree was constructed using the Neighbor-Joining (NJ) method and phylogenetic analysis. 【Result】 The average intraspecific genetic distance of 16 whiteflies was 0.0015, the average genetic distance was 0.2897, and the interspecific genetic distance was 193.1 times of the intraspecific genetic distance when the 5 ’end was targeted. There is no overlap between the genetic distances between species. The intraspecific genetic distance was 0.0007 when the 3 ’end was targeted. The average genetic distance between the two species was 0.2817. The genetic distance was 402.4 times of the intraspecific genetic distance. However, Pealius mori and Bemisia tabaci Asia The intraspecies and interspecific genetic overlap of II 1 overlapped. The results of phylogenetic analysis showed that 16 whiteflies could form independent evolutionary branches when the 5 ’end was the target. When the 3’ end was the target, all the other species could be formed independent of the traditional taxonomy Branch. 【Conclusion】 The results show that the 5 ’end sequence is more suitable for identification of species based on DNA barcoding technology.