论文部分内容阅读
目的建立稳定的9L/F344大鼠脑胶质瘤模型。方法15只近交系雄性F344大鼠,采用立体定向技术,在大鼠右侧额叶尾状核接种1×105个9L细胞,观察大鼠生存状态;接种后第10、20天行MRI检查,观测颅内肿瘤生长情况。大鼠死亡后,取脑制作病理切片,行HE染色后观察肿瘤组织形态,采用免疫组织化学方法检测胶质纤维酸性蛋白(GFAP)和S-100蛋白。结果大鼠接种肿瘤细胞后30d内全部死亡,平均生存时间(24.4±3.3)d。接种后10d,MRI增强扫描即可见肿瘤生成;接种后20d,MRI检查可见接种侧脑组织大片水肿,增强扫描肿瘤显像清晰。脑内均见肿瘤形成,未见肿瘤颅外生长;肿瘤周边界线较明显,但未见包膜;瘤内新生血管丰富,可见出血、坏死;肿瘤免疫组织化学GFAP和S-100蛋白染色阴性。结论该方法建立的大鼠脑胶质瘤模型稳定可靠,符合恶性胶质肉瘤生物学特性。该模型是理想的脑胶质瘤实验研究材料。
Objective To establish a stable 9L / F344 rat glioma model. METHODS: Fifteen inbred male F344 rats were inoculated with 1 × 105 9L cells on the right frontal lobe of caudate nucleus using stereotactic technique to observe the survival of rats. MRI examination was performed on the 10th and 20th day after inoculation , Observation of intracranial tumor growth. After the death of the rats, brain sections were taken for pathological examination. The morphology of the tumor was observed by HE staining. The glial fibrillary acidic protein (GFAP) and S-100 protein were detected by immunohistochemistry. Results All rats died within 30 days after inoculation of tumor cells, with an average survival time of (24.4 ± 3.3) d. On the 10th day after inoculation, the tumors could be seen by MRI enhanced scanning. On the 20th day after inoculation, large edema of the inoculated side brain tissue was observed by MRI, and the appearance of enhanced tumor imaging was clear. Tumor formation was seen in the brain, no tumor extracranial growth; tumor perimeter boundaries more obvious, but no envelope; tumor neovascularization rich, visible bleeding, necrosis; tumor immunohistochemical GFAP and S-100 protein staining negative. Conclusion The rat glioma model established by this method is stable and reliable, which is in line with the biological characteristics of glioblastoma. The model is an ideal brain glioma experimental study material.