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合成了两种2-氨甲基苯并咪唑(ambi)和去甲基斑蝥酸(H2DCA=7-氧杂二环[2.2.1]庚烷-2,3-二甲酸)与镨(Ⅲ)、钐(Ⅲ)的配合物。应用元素分析、摩尔电导、红外光谱及X射线单晶衍射法对配合物的组成和结构进行了表征,配合物的组成为:(Hambi)[Ln(DCA)2(H2O)3]·3H2O(Ln=Pr(III)(1),Sm(III))(2);Hambi为质子化的ambi。DCA离子的醚键和羧酸根的氧原子参与配位,为三齿配体,稀土离子的配位数为9。通过紫外光谱法、荧光光谱法和粘度法研究了配合物与DNA和牛血清白蛋白(BSA)的相互作用。结果表明:配合物能通过部分插入模式与DNA发生较强的结合作用(Kb:1.56×104(1)和1.85×104L·mol-1(2))。配合物能与BSA发生强烈的相互作用(KA:9.33×104L·mol-1(1)和1.52×106L·mol-1(2)),结合位点数为1。测试了配合物对人肝癌细胞(SMMC7721)的体外抗增殖活性。结果显示,配合物的抗癌活性较去甲基斑蝥素有明显提高。
Two kinds of 2-aminomethylbenzimidazole and demethyl cantharidic acid (H2DCA = 7-oxabicyclo [2.2.1] heptane-2,3-dicarboxylic acid) , Samarium (III) complexes. The composition and structure of the complex were characterized by elemental analysis, molar conductance, IR and single crystal X-ray diffraction. The composition of the complex was as follows: (Hambi) [Ln (DCA) 2 (H2O) 3] Ln = Pr (III) (1), Sm (III)) (2); Hambi is a protonated ambi. The ether bond of DCA and the oxygen atom of carboxylate participate in the coordination, which is a tridentate ligand. The coordination number of rare earth ions is 9. The interaction of the complex with DNA and bovine serum albumin (BSA) was studied by UV spectroscopy, fluorescence spectroscopy and viscometry. The results showed that the complexes could strongly bind DNA with partial insertion patterns (Kb: 1.56 × 104 (1) and 1.85 × 104L · mol-1 (2)). The complexes could strongly interact with BSA (KA: 9.33 × 104 L · mol-1 (1) and 1.52 × 106 L · mol-1 (2)) with a binding site number of 1. The in vitro anti-proliferative activity of the complex on human hepatocellular carcinoma cells (SMMC7721) was tested. The results showed that the anticancer activity of the complex was significantly higher than that of norcantharidin.