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以环氧氯丙烷作为多功能官能团交联剂，经化学合成反应，将β－环糊精固载在活性轻质二氧化硅表面上，以此为固定相制备成层析薄板，直接用于拆分氨基酸的Ｄ，Ｌ－对映异构体．实验结果表明：该层析薄板在脱氧胆酸或尿素的协同作用下，可用于拆分亲脂性基因与手性碳直接相连的氨基酸Ｄ，Ｌ－对映体．研究了在展开剂中不同浓度的脱氧胆酸和尿素两种立体选择助剂对这３种氨基酸的Ｄ，Ｌ－对映异构体的分离效果的影响，并对其机理进行了初步讨论．
Epichlorohydrin as a multifunctional functional group crosslinking agent, the chemical synthesis reaction, the β-cyclodextrin immobilized on the surface activity of light silica, as a stationary phase prepared into a thin layer of chromatography, used directly Split the D, L-enantiomer of the amino acid. The experimental results show that this chromatographic plate can be used to resolve the D, L-enantiomers of amino acids directly linked to the chiral carbon with lipoxygenase under the synergistic effect of deoxycholic acid and urea. The effects of different concentrations of deoxycholic acid and urea on the separation of D, L-enantiomers of these three amino acids were studied. The mechanism was also discussed.