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目的:探讨内皮细胞型一氧化氮合酶基因(ecNOS)第7号外显子G894T多态性与前列腺癌是否存在相关性。方法:取78例前列腺癌患者和88例前列腺增生患者外周血4 ml,提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测ecNOS基因G894T多态性,分析两组ecNOS基因G894T的基因型频率与等位基因频率的差异。结果:ecNOS基因G894T存在G、T2种等位基因以及GG、GT、TT3种基因型;前列腺癌组与前列腺增生组相比,ecNOS基因G894T的基因型频率及等位基因频率差异均无统计学意义(P>0.05)。结论:ecNOS基因G894T多态性可能不是前列腺癌发病的遗传危险因素。
Objective: To investigate whether there is a correlation between exon 7 G894T polymorphism of endothelial nitric oxide synthase gene (ecNOS) and prostate cancer. Methods: Genomic DNA was extracted from 4 cases of peripheral blood from 78 patients with prostate cancer and 88 patients with benign prostatic hyperplasia. The genotypes of G894T polymorphism in ecNOS gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The difference of genotype frequency and allele frequency between two groups of ecNOS gene G894T was analyzed. Results: There were G and T2 alleles and GG, GT and TT genotypes in ecNOS gene G894T. There was no significant difference in genotype and allele frequencies of ecNOS gene G894T between prostate cancer group and benign prostatic hyperplasia group Significance (P> 0.05). Conclusion: The G894T polymorphism of ecNOS gene may not be a genetic risk factor for the development of prostate cancer.