Application of LC–MS/MS for quantitative analysis of glucocorticoids and stimulants in biological fu

• 作 者: Jamshed Haneef Mohammad Shaharyar Asif Husain Mohd Rashid Ravinesh Mishra Shama Parveen Niyaz Ahmed Manoj Pal Deepak Kumar
• 机 构: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University),Depa
• 出 处: Journal of Pharmaceutical Analysis
• 发表日期: 2013年05期
• 关键词: selectivity fragmentation pharmaceutical packing focusing doping meant urine met

Liquid chromatography tandem mass chromatography(LC–MS/MS)is an important hyphenated technique for quantitative analysis of drugs in biological fuids.Because of high sensitivity and selectivity,LC–MS/MS has been used for pharmacokinetic studies,metabolites identifcation in the plasma and urine.This manuscript gives comprehensive analytical review,focusing on chromatographic separation approaches(column packing materials,column length and mobile phase)as well as different acquisition modes(SIM,MRM)for quantitative analysis of glucocorticoids and stimulants.This review is not meant to be exhaustive but rather to provide a general overview for detection and confrmation of target drugs using LC–MS/MS and thus useful in the doping analysis,toxicological studies as well as in pharmaceutical analysis.

LC-MS / MS has been used for pharmacokinetic studies, metabolites identifcation in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. to be exhaustive but rather to provide a general overview for detection and confrmation of target drugs using LC-MS / MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.