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为了研究使用甲型肝炎灭活疫苗快速免疫美军士兵的可行性,作者将276名血清阴性的士兵随机分成4组,分别于0、0天,0、14天,0、30天和0、180天接种两剂福马林灭活的甲型肝炎疫苗,每剂1ml,含720 ELISA单位(ELU)。第380天再接种第3剂疫苗。分别在0、14、30、60、180、240、380、395、410和560天采血检测丙氨酸转氨酶(ALT)水平,用定量ELISA、放射免疫灶抑制试验(RIFIT)及改良放射免疫测定(RIA)法分别检测血清抗甲型肝炎病毒(HAV)抗体和中和抗体,并用中和试验检测抗体中和一些HAV株的能力,然后用统计学方法分析抗体几何平均浓度
In order to investigate the feasibility of using a Hepatitis A inactivated vaccine for rapid immunization of U.S. soldiers, 276 seronegative soldiers were randomly assigned to 4 groups at 0, 0, 0, 14, 0, 30 and 0, 180 Inoculated with two doses of famcr-1 inactivated hepatitis A vaccine, 1 ml per dose, contains 720 ELISA units (ELU). Day 380 The third dose of vaccine was inoculated. The levels of alanine aminotransferase (ALT) were determined at 0,14,30,60,180,240,380,395,410 and 560 days respectively. The levels of ALT were measured by quantitative ELISA, radioimmunoassay (RIFIT) and modified radioimmunoassay (RIA) method were used to detect the serum anti-HAV antibody and neutralizing antibody respectively. The neutralization test was used to test the ability of the antibody to neutralize some HAV strains, and then the antibody geometric mean concentration