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目的观察供者来源的未成熟树突状细胞(imDCs)联合骨髓移植(BMT)对大鼠移植肾的保护作用,并探讨其机制。方法DA(RT1a)大鼠为供者,Lewis(RT1l)大鼠为受者,Wistar大鼠为无关第三品系。将实验动物随机分为5组,每组8只,进行不同的预处理后进行肾移植。(1)阴性对照组:受者不接受任何预处理;(2)imDCs诱导组:受者术前7d经尾静脉注射经60Co照射(30 Gy)灭活的、供者来源的未成熟树突状细胞2×107/只;(3)BMT诱导组:受者术前4 d经尾静脉注射供者来源的新鲜骨髓细胞2×108/只;(4)imDCs+BMT联合诱导组:受者术前7 d经尾静脉注射经60Co照射(30 Gy)灭活的、供者来源的未成熟树突状细胞2×107/只,术前4 d经尾静脉注射供者来源的新鲜骨髓细胞2×108/只;(5)第三品系组:预处理与imDCs+BMT联合诱导组相同,但供者肾脏来自Wistar大鼠。术后进行单向混合淋巴细胞反应(MLR);白细胞介素2(IL-2)逆转实验;体内细胞转移实验(DTH);流式细胞仪检测RT1a阳性细胞百分率。结果各组大鼠肾移植后平均存活时间分别为:阴性对照组(7.12±1.25)d;imDCs诱导组(24.38±3.20)d;BMT诱导组(7.87±2.10)d;第三品系组(6.63±1.06)d;而imDCs+BMT联合诱导组延长到(80.75±16.88)d;后者与上述4组比较,差异均有统计学意义(P<0.01)。免疫耐受的大鼠脾细胞增殖?
Objective To observe the protective effect of donor immature DCs (imDCs) combined with bone marrow transplantation (BMT) on renal allograft in rats and its mechanism. Methods DA (RT1a) rats were donors, Lewis (RT1l) rats as recipients, Wistar rats as unrelated third strains. The experimental animals were randomly divided into 5 groups, 8 in each group. After different pretreatment, kidney transplantation was performed. (1) Negative control group: recipients did not receive any pretreatment; (2) imDCs induced group: recipients were injected via the tail vein 60 min before irradiation (30 Gy), donor-derived immature dendrites (3) BMT induction group: recipients were injected 2 × 108 fresh myeloid cells via caudal vein 4 days before operation; (4) imDCs + BMT induction group: recipients Seven days before operation, 2 × 107 immature dendritic cells inactivated by 60Co irradiation (30 Gy) were injected into the caudal vein through the caudal vein, and fresh myeloid cells 2 × 108 / mouse; (5) Tertiary strain group: The pretreatment group was the same as the imDCs + BMT group, but the donor kidney was from Wistar rats. One-way mixed lymphocyte reaction (MLR) was performed postoperatively. Interleukin 2 (IL-2) reversal assay, in vivo cell transfer assay (DTH) and flow cytometry were used to detect the percentage of RT1a positive cells. Results The renal mean survival time after transplantation were: negative control group (7.12 ± 1.25) d; imDCs treated group (24.38 ± 3.20) d; BMT treated group (7.87 ± 2.10) d; a third set of lines ( 6.63 ± 1.06) d, while the combination of imDCs + BMT induced group was prolonged to (80.75 ± 16.88) d; the latter was significantly different from the above 4 groups (P <0.01). Immune tolerance of rat spleen cell proliferation?