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用免疫组织化学方法研究了神经激肽B免疫反应纤维在实验性哮喘豚鼠下呼吸道的变化。在气管和支气管,实验组一抗最佳稀释浓度为1:1500,对照组为1:1000,当两组的一抗浓度均为1:1500时,两者的神经激肽B免疫反应纤维见于平滑肌层,上皮内、上皮和平滑肌交界处以及外膜层,实验组的纤维数量明显多于对照组。在肺组织,实验组一抗最佳稀释浓度为1:2000,对照组为1:1500,当一抗稀释浓度均为1:2000时,两组的神经激肽B免疫反应纤维主要见于肺内各级支气管平滑肌层、血管壁平滑肌层、血管周围结缔组织和肺泡隔内,但实验组的阳性纤维数量明显比对照组多。
Immunohistochemistry was used to investigate the changes of neurokinin B immunoreactive fibers in the lower respiratory tract of experimental asthmatic guinea pigs. In the trachea and bronchus, the optimal dilution of the primary antibody in the experimental group was 1: 1500 and in the control group was 1: 1000. When the primary antibody concentration in both groups was 1: 1500, both of the neurokinin B immunoreactive fibers were found in Smooth muscle layer, epithelial, epithelial and smooth muscle junction and the outer membrane, the experimental group, the number of fibers was significantly more than the control group. In lung tissue, the optimal dilution of the primary antibody in the experimental group was 1: 2000 and in the control group was 1: 1500. When the primary antibody dilution was 1: 2000, the neurokinin B immunoreactive fibers in the two groups were mainly found in the lung All levels of bronchial smooth muscle layer, smooth muscle layer of vascular wall, perivascular connective tissue and alveolar septum, but the experimental group, the number of positive fibers significantly more than the control group.