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目的:对小檗的组织构造与显微特征进行观察,为其资源的进一步开发利用以及品质评价提供科学依据。方法:1采用石蜡切片法制备小檗根、茎和叶的组织切片,观察其组织构造及特征。2采用水合氯醛透化或醋酸甘油不透化法制备粉末片,观察粉末的显微特征。结果:1小檗根横切片皮层有石细胞散在,单个或成群,韧皮部及中柱鞘可见纤维。小檗根粉末中中柱鞘纤维长梭形(320.8~378.3μm),壁厚,木化,孔沟清晰,淡黄色或黄绿色;石细胞类方形、类圆形或不规则形,壁厚,孔沟清晰,黄棕色或黄绿色。2茎横切片的皮层可见少量石细胞,韧皮部有石细胞群伴有纤维。小檗茎粉末中中柱鞘纤维细长梭形(283.5~396.6μm),壁厚,木化,淡黄色或黄绿色;石细胞类长方形、类圆形或不规则形,壁厚,孔沟清晰,黄绿色或淡黄棕色。3叶横切片上表皮细胞长方形,其下有栅栏细胞1列;下表皮细胞细小扁平,可见气孔;主脉维管束外韧型,上下均有微木化的纤维束。小檗叶粉末中表皮细胞多角形,细胞壁不均匀增厚;气孔不定式;单细胞非腺毛圆锥形,壁薄,有轻微疣状突起,基部弯曲。结论:小檗根、茎和叶的组织构造与粉末的显微特征比较明显,可为该药材的生药学研究和质量控制提供参考或依据。
OBJECTIVE: To observe the microstructure and microscopic features of Berberis and provide a scientific basis for the further development and utilization of its resources and its quality evaluation. Methods: 1 The paraffin sections were used to prepare the tissue sections of the roots, stems and leaves of the barberry. The tissue structure and characteristics were observed. 2 using chloral hydrate or glycerol glycerol impermeable preparation of powder tablets, observe the microscopic characteristics of the powder. Results: 1 Berberis root transverse section of the cortex scattered stone cells, single or groups, phloem and pericycle column fibers. Berberis root powder in the long columnar sheath sheath fibers (320.8 ~ 378.3μm), wall thickness, woody, clear ditch, yellow or yellowish green; stone cells square, round or irregular shape, wall thickness , Clear ditch, yellow-brown or yellow-green. 2 stalk transverse section of the cortex shows a small number of stone cells, phloem with stone cell group with fiber. Berber stem powder in the columnar sheath fibers elongated fusiform (283.5 ~ 396.6μm), wall thickness, woody, yellowish or yellowish green; stone cells rectangular, round or irregular, wall thickness, hole ditch Clear, yellowish green or yellowish brown. 3 leaf transverse section of the epidermal cells rectangular, there is a column of palisade cells under it; the lower epidermal cells flat and small, visible stomata; main vein vascular bundle tough type, both upper and lower micro-wood fiber bundles. Berberis leaf powder in the epidermal cells polygonal, uneven cell wall thickening; infiniform stomatal; single-cell non-glandular hairy conic, thin wall, with slight verrucous protrusions, base curved. Conclusion: The microstructure of the roots, stems and leaves of the barberry is more obvious than that of the powder, which may provide a reference or basis for the pharmacognosy research and quality control of the medicinal materials.