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目的 :建立了同时测定益肺佳营养液中维生素C和维生素B6含量的方法。方法 :高效液相色谱法。固定相为十八烷基硅烷键合硅胶 ;流动相为 0 .0 1mol/L磷酸二氢钾溶液 (用磷酸调节PH至 2 .5)。检测波长 :①维生素C紫外检测 ,波长 2 4 2nm ;②维生素B6荧光检测 ,激发波长 2 90nm ,发射波长 395nm。结果 :线性范围 :维生素C 2 0 .5～1 0 2 .3μg/ml,r =0 .9999;维生素B60 .2 8～ 1 .4 2 μg/ml,r=0 .9999。方法回收率 :维生素C为 99.1 %,n =5,RSD为0 .4 8%;维生素B6为 1 0 0 .2 %,n =5,RSD为 1 .2 1 %。结论 :该方法操作简便快速 ,灵敏度高 ,结果准确 ,适用于该制剂中维生素C和维生素B6的含量测定。
Objective: To establish a method for simultaneous determination of vitamin C and vitamin B6 in Yifeijia nutrient solution. Method: HPLC. The stationary phase was octadecylsilane-bonded silica; the mobile phase was a 0.1% potassium dihydrogen phosphate solution (pH was adjusted to 2.5 with phosphoric acid). Detection wavelength: 1 vitamin C UV detection, wavelength 342nm; 2 vitamin B6 fluorescence detection, excitation wavelength 2 90nm, emission wavelength 395nm. Results: Linear range: Vitamin C 2 0.5 to 10 2 3 μg/ml, r =0.9999; Vitamin B 60 0.28 to 1.42 μg/ml, r=0.9999. The recovery of the method: vitamin C was 99.1 %, n = 5, RSD was 0.48%; vitamin B6 was 10.0%, n = 5, and RSD was 1.21%. Conclusion : This method is simple, rapid, sensitive, and accurate. It is suitable for the determination of vitamin C and vitamin B6 in this preparation.