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目的:观察缺血/再灌注损伤肠道Cajal间质细胞、炎症因子、氧自由基变化以及拟胆碱药卡巴胆碱对上述指标的影响。方法:成年雄性SD大鼠,随机分为手术对照(S)组、肠缺血再灌注(I/R)组和I/R+卡巴胆碱(I/R+CAR)组。制备肠I/R模型,于夹闭后2.5 h处死动物,取肠袋组织测定TNF-α含量、SOD活性和MDA含量;另取肠组织制作病理切片、进行Cajal间质细胞免疫组化染色。结果:I/R组肠组织中Cajal间质细胞阳性表达量比S组显著降低(P<0.01),I/R+CAR组Cajal间质细胞阳性表达量显著高于I/R组(P<0.01)。I/R组MDA和TNF-α含量较S组显著升高(P<0.01)、SOD活性显著降低(P<0.01)。I/R+CAR组MDA和TNF-α含量与I/R组比较明显减少(P<0.01),SOD活性有所回升(P<0.05)。结论:肠缺血/再灌注损伤时炎症因子及氧自由基增加,导致Cajal间质细胞损伤;卡巴胆碱可通过减少炎症因子释放及氧自由基损伤,减轻Cajal间质细胞的损伤。
OBJECTIVE: To observe the changes of intestinal Cajal interstitium, inflammatory cytokines and oxygen free radicals in ischemia / reperfusion injury and the effects of choline chloride, a cholinergic drug, on these indexes. Methods: Adult male Sprague-Dawley rats were randomly divided into operation control group (S), intestinal ischemia reperfusion (I / R) group and I / R + carbachol (I / R + CAR) group. The intestine I / R model was established. The animals were sacrificed 2.5 h after the occlusion, and the contents of TNF-α, SOD and MDA were determined in the intestine tissue. Pathological sections of intestinal tissue were made for Cajal interstitial cells immunohistochemical staining. Results: The positive expression of Cajal interstitial cells in I / R group was significantly lower than that in S group (P <0.01), and the positive expression of Cajal interstitial cells in I / R + CAR group was significantly higher than that in I / R group (P < 0.01). The contents of MDA and TNF-α in I / R group were significantly higher than those in S group (P <0.01), while the activity of SOD was decreased significantly (P <0.01). The contents of MDA and TNF-α in I / R + CAR group were significantly decreased compared with those in I / R group (P <0.01), while SOD activity was increased (P <0.05). CONCLUSION: Inflammatory cytokines and oxygen free radicals are increased during intestinal ischemia / reperfusion injury, resulting in Cajal interstitial cell injury. Carbachol may reduce the injury of Cajal interstitium by reducing the release of inflammatory cytokines and oxygen free radicals.