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OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.
OBJECTIVE: To observe the effects of different doses of Wenxiao II Decoction on the expression of monocyte chemoattractant protein-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis. METHODS: Sixty 3-4 month-old New Zealand rabbits of both sexes were differentiated into six groups: simvastain; model; blank; and high-dose, mid- dose, and low-dose Wenxiao Ⅱ Decoction groups. Except for those in the blank group, all rabbits were fed a high-cholesterol diet. Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet. After All rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining .RESULTS: Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group ( P <0 .05) .When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable, and the differences were indistinct (P <0.05) .When comparing among Wenxiao II Decoction groups, MCP-1 expression in the mid-and high-dose groups were significantly lower than that seen in the low-dose group (P <0.01), but there were no differences among three dosage groups with respect to VCAM-1 expression (P> 0.05) .CONCLUSION: These data suggest that high, mid, and low doses of Wenxiao II Decoction can inhibit the expression of MCP-1 and VCAM-1, which may prevent the formation of or stabilize atherosclerotic plaques. Here may be a direct relationship between the dosage of Wenxiao II Decoction and its therapeutic efficacy.