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目的:探讨COX-2和TGF-β1蛋白在乳腺癌变过程中的表达变化情况与意义。方法:采用免疫组化即用型二步法(非生物素)染色方法检测乳腺正常组织(61例)、乳管内乳头状瘤(65例)、原位癌(45例)及乳腺浸润性癌(87例)乳腺组织中COX-2、TGF-β1蛋白的表达情况。结果:总体表达情况上COX-2在乳头状瘤组织(6.26±2.56)、原位癌组织(7.23±3.02)及浸润性癌组织的表达(5.90±2.22)与正常乳腺上皮组织(4.73±2.03)相比,差异有统计学意义,P<0.01;乳头状瘤组织(2.15±0.97)与原位癌组织(2.52±1.18)的表达强度高于浸润性癌组织(1.68±0.86),P<0.01。TGF-β1总体表达情况在乳头状瘤组织(5.76±2.21)、原位癌组织(7.08±3.13)及浸润性癌组织的表达(6.77±2.54)与正常乳腺上皮组织的表达(4.88±2.13)相比,差异均有统计学意义,P<0.05;原位癌组织(2.23±1.16)及浸润性癌组织(2.21±1.09)表达强度高于乳管内乳头状瘤组织(2.01±0.91)与正常乳腺上皮组织(1.87±0.84),P<0.05。结论:COX-2及TGF-β1蛋白表达可能是乳腺癌癌变的早期事件,检测可能有助于临床的早期诊断及预防。
Objective: To investigate the expression of COX-2 and TGF-β1 in breast carcinogenesis and its significance. Methods: Immunohistochemical two-step immunohistochemical (non-biotin) staining was used to detect normal breast tissue (61 cases), intraductal papilloma (65 cases), carcinoma in situ (45 cases) and invasive breast cancer (87 cases) breast cancer tissue COX-2, TGF-β1 protein expression. Results: The overall expression of COX-2 in normal breast epithelium (4.73 ± 2.03) was significantly higher than that in normal breast epithelium (6.26 ± 2.56), carcinoma in situ (7.23 ± 3.02) and invasive carcinoma (5.90 ± 2.22) (P <0.01). The expression intensity of papillary tumor (2.15 ± 0.97) and carcinoma in situ (2.52 ± 1.18) was higher than that of invasive cancer (1.68 ± 0.86), P < 0.01. The overall expression of TGF-β1 was significantly higher in normal breast epithelial tissues (4.88 ± 2.13) than that in normal breast epithelial tissues (5.76 ± 2.21, 7.08 ± 3.13, 6.77 ± 2.54, (2.23 ± 1.16) and invasive cancer (2.21 ± 1.09), respectively, which were significantly higher than those in the ductal papilloma (2.01 ± 0.91) and normal Breast epithelium (1.87 ± 0.84), P <0.05. CONCLUSION: The expression of COX-2 and TGF-β1 may be early events of breast cancer. Detection may be helpful for the early diagnosis and prevention of breast cancer.