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本文选用普氏立克次体柠檬酸合成酶基因做为靶序列合成一对引物（ＲＰＣＳ８７７ｐ和ＲＰＣＳ１２５８ｎ）。用该对引物对不同种类立克次体（包括：加拿大；普氏；莫氏；Ｑ热；恙虫病Ｇｉｌｌｉａｍ株、Ｋａｔｏ株、Ｋａｒｐ株；澳大利亚；立氏；康氏；小蛛；西伯利亚２４６、西伯利亚２３２；精河；内０１；内Ｗ８８；黑８４；黑０５４）及大肠杆菌ＨＢ１０１，ＤＮＡ进行扩增。同时，选用三种不同方法处理模板进行扩增结果的比较。结果表明：该引物可扩增除恙虫病以外的所有立克次体，而大肠杆菌、λＤＮＡ不能被扩增。采用简单煮沸法处理模板，对含立克次体的材料进行扩增是可行的。
In this paper, a pair of primers (RPCS877p and RPCS1258n) were synthesized using the gene encoding Phytase of Platycodes rickettsia. The pair of primers was used to test the abundance of different species of Rickettsia (including: Canada; Platts; Morse; Q fever; Gilliam strain, Kato strain, Karp strain; Australia; Siberia 232; Jinghe; inner 01; inner W88; black 84; black 054) and E. coli HB101, DNA. At the same time, three different methods were used to process the template to compare the amplification results. The results showed that this primer could amplify all Rickettsia except scrub typhus, while λDNA of E. coli could not be amplified. It is feasible to use simple boiling method to process the template and to amplify the material containing rickettsia.