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AIM:To assess the effects of extract of Buddleja officinalis on tear secretion volume,tear film stability,expressions of TGF-β1,IL-1β,TNF-αin lacrimal gland of castrated rabbits with dry eye. METHODS:A total of 30 victory rabbits were divided averagely into normal group(A),model group(B),therapy group with low dose extract of Buddleja officinalis(C), therapy group with high dose extract of Buddleja officinalis (D)and therapy group with genistein(E).The dry eye model was established with orchiectomy on Group B,C,D,E.Group C,D,E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT.TGF-β1,IL-1β,TNF-αwere detected with immunohistochemistry and the ultrastructure of lacrimal gland was observed under transmission electron microscope. RESULTS:The SIT value of group C,D,E were respectively 13.167±4.957,14.667±5.279,8.667±0.516,obviously higher than that of group B 5.667±2.338(P<0.01).The positive expression of IL-1βin acinar cell and glandular tube cell of group C,D were 0.470±0.048,0.510±0.088, obviously lower than that of group B 0.770±0.118(P<0.01). The positive expression of TNF-αof group C,D were 0.498±0.156,0.435±0.069,obviously lower than that of group B 0.769±0.095 too(P<0.01).The positive expression of TGF-β1 of group C,D were 0.406±0.171,0.497±0.147, obviously higher than that of group B 0.222±0.113(P<0.01). Any result of group C,D was positive compared with that of group E(P<0.05).Ultrastructure of the lacrimal gland of group C,D,E was well preserved,especially in D group it was remarkable. CONCLUSION:The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.
AIM: To assess the effects of extract of Buddleja officinalis on tear secretion volume, tear film stability, expressions of TGF-β1, IL-1β, TNF-αin lacrimal gland of castrated rabbits with dry eye. METHODS: A total of 30 victory rabbits were divided averagely into normal group (A), model group (B), therapy group with low dose extract of Buddleja officinalis (C), therapy group with high dose extract of Buddleja officinalis (D) and therapy group with genistein (E). The dry eye model was established with orchiectomy on Group B, C, D, E. Group C, D, E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT.TGF-β1 RESULTS: The SIT value of group C, D, E respectively was 13.167 ± 4.957, 14.667 ± 5.279, 8.667 ± 0.516, obviously higher than that of group B 5.667 ± 2.338 (P <0.01) .Th e positive expression of IL-1βin acinar cell and glandular tube cell of group C, D were 0.470 ± 0.048, 0.510 ± 0.088, obviously lower than that of group B 0.770 ± 0.118 (P <0.01). The positive expression of TNF- group C, D were 0.498 ± 0.156, 0.435 ± 0.069, obviously lower than that of group B 0.769 ± 0.095 too (P <0.01). The positive expression of TGF-β1 of group C, D were 0.406 ± 0.171, 0.497 ± 0.147 , obviously higher than that of group B 0.222 ± 0.113 (P <0.01). Any result of group C, D was positive compared with that of group E (P <0.05). Ultrstructure of the lacrimal gland of group C, D, E was well preserved, especially in D group it was remarkable. CONCLUSION: The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.