论文部分内容阅读
目的建立针对非结核分枝杆菌中瘰疬分枝杆菌菌株的real-time PCR检测方法。方法在我们的研究中,根据瘰疬分枝杆菌的SodA基因设计MGB探针和特定的引物,并用来检测模拟样品中的瘰疬分枝杆菌。结果该方法最低瘰疬分枝杆菌DNA检测浓度是101拷贝数,标准曲线显示阈值周期和SodA基因片段拷贝数之间的相关系数是0.973,斜率为-3.249,表现出良好的线性关系。此外,模拟样品最低检测浓度是每毫升101个细菌,此外,其他9株菌为阴性结果,验证了良好的特异性。结论该方法对于检测瘰疬分枝杆菌模拟标本显示很高的敏感性和特异性,可用于瘰疬分枝杆菌菌株的生态和流行病学监测。
Objective To establish a real-time PCR detection method for Mycobacterium reyclonicus strains in non-tuberculous mycobacteria. Methods In our study, MGB probes and specific primers were designed based on the SodA gene of Mycobacterium bovis and used to detect M. bovis in mock samples. Results The minimum detection concentration of Mycobacterium bovis DNA was 101 copies. The standard curve showed that the correlation coefficient between the threshold cycle and the SodA gene copy number was 0.973 and the slope was -3.249, showing a good linear relationship. In addition, the minimum detectable concentration of the simulated samples was 101 bacteria per ml, and the other 9 strains were negative, demonstrating good specificity. Conclusion This method is very sensitive and specific for the detection of Mycobacterium bovis mimic and can be used for ecological and epidemiological surveillance of Mycobacterium bovis strains.