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目的建立分散固相萃取-表面增强拉曼光谱法(dispersive solid phase extraction-surface enhanced raman scattering,d-SPE-SERS)快速检测蔬菜中10种有机磷农药残留的分析方法。方法采用分散固相萃取对豇豆样品进行前处理,结合表面增强拉曼光谱技术进行检测并进行方法学验证。结果 10种有机磷农药在0.2~15μg/m L的质量浓度范围内,与相应的特征峰强度呈良好的线性关系,相关系数在0.9863~0.9995之间,检出限范围为0.2~1μg/m L,平均加标回收率在59.3%~100.1%之间,RSD≤11.2%(n=5)。结论该方法具有操作简单、迅速、准确性高等特点,适用于豆类等蔬菜中多种有机磷农药残留的快速筛查及鉴定。
OBJECTIVE To establish a method for rapid determination of 10 organophosphorus pesticide residues in vegetables by dispersive solid phase extraction-surface enhanced raman scattering (d-SPE-SERS). Methods The cowpea samples were pretreated with dispersive solid phase extraction (SPE) and detected by surface enhanced Raman spectroscopy (SERS). Results The results showed that there was a good linear relationship between the ten organophosphorus pesticides and the corresponding peak intensities in the range of 0.2-15 μg / mL, the correlation coefficient was between 0.9863 and 0.9995, and the detection limit was 0.2-1 μg / m L, the average recovery was between 59.3% and 100.1%, RSD≤11.2% (n = 5). Conclusion The method is simple, rapid and accurate. It is suitable for the rapid screening and identification of a variety of organophosphorus pesticide residues in vegetables such as beans.