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作者观察到大萼金丝桃(Hypericumcalycinum L.)地上部分的石油醚提取物显示了重要的抑制Co-115人结肠癌细胞系生长的活性。原亲脂性提取物经DCCC,石油醚-94％乙醇-乙酸乙酯-水(83:67:33:17,上行法)分出了3个有活性的部分。主要活性部分从己烷中结晶,再经RP-18半制备HPLC,甲醇-水(85:15)+0.1％乙酸,得到化合物(1)和(2)。与早先从金丝桃(H.chinense L.)中分出的抗菌素金不换素(chinensin)Ⅰ和Ⅱ相比,(1)与金不换
The authors observed that the petroleum ether extract of aerial parts of Hypericum calycinum L. showed significant inhibition of the growth of Co-115 human colon cancer cell line. The original lipophilic extract was separated into three active fractions by DCCC, petroleum ether-94% ethanol-ethyl acetate-water (83:67:33:17, ascending method). The main active moiety was crystallized from hexane and subjected to RP-18 semi-preparative HPLC, methanol-water (85:15) + 0.1% acetic acid to give compounds (1) and (2). Compared with the antibiotic golds (Kinensin) I and II that were separated from H.chinense L. earlier,