虎杖中大黄酚、大黄素甲醚提取与分离的实验

• 作 者: 何祖鞭
• 机 构: 安徽和县人民医院
• 出 处: 中成药研究
• 发表日期: 1986年02期
• 关键词: 大黄素甲醚 大黄酚 虎杖 石油醚 洗脱剂 萃取法 实验 脱水物 大黄酚标准品 硅胶柱层析

用柱层析法分离大黄酚和大黄素甲醚,国内大多沿用传统的含苯溶剂进行洗脱。由于苯毒性大,影响人体健康。本文报道了从虎杖中提取和分离大黄酚与大黄素甲醚的方法。实验先采用梯度pH萃取法,然后以石油醚-醋酸乙酯(96:4)作硅胶柱层析洗脱剂,取得了较好的分离效果。笔者认为,此法毒性小,分离效果好。使用方便。

Separation of chrysophanol and emodin by column chromatography, most of the domestic use of traditional benzene-containing solvents for elution. As benzene is toxic, it affects human health. This article reports on the extraction and separation of chrysophanol and rhubarb monomethyl ether from Polygonum cuspidatum. Gradient pH extraction was used firstly, and then petroleum ether-ethyl acetate (96:4) was used as silica gel column elution agent to obtain better separation effect. The author believes that this method has little toxicity and good separation effect. Easy to use.