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[目的]明确交配和黑光灯处理对棉铃虫生物钟基因cryptochromes mRNA表达的影响。[方法]应用实时定量PCR(SYBRGreen)技术检测不同条件下棉铃虫cryptochromes(cry1和cry2)基因的表达。提取棉铃虫头部总RNA,经DNaseI消化后进行反转录合成cDNA,并采用特异性引物分别对cry1、cry2和EF-1α基因进行实时定量PCR扩增。[结果]黑光灯处理棉铃虫2h,其cry1mRNA的表达量明显降低;cry2mRNA的表达量小于对照,但两者之间差异性并不显著。交配对棉铃虫cry1和cry2 mRNA表达均存在显著影响,并且雌、雄两性cry1和cry2mRNA表达量在交配后随时间延长呈下降趋势。[结论]该结果对进一步研究cry基因的功能以及棉铃虫防治具有重要意义。
[Objective] The research aimed to clarify the effects of mating and black light on the expression of the cryptochromes mRNA in the clock of the cotton bollworm (Helicoverpa armigera). [Method] The expression of cryptochromes (cry1 and cry2) genes of Helicoverpa armigera were detected by real-time quantitative PCR (SYBRGreen). The total RNA was extracted from the head of cotton bollworm, digested with DNaseI, and reverse transcribed into cDNA. The specific primers were used to amplify the genes cry1, cry2 and EF-1α respectively. [Result] The cry1mRNA expression of cotton bollworm treated with black light was significantly lower than that of the control, but the difference between the two was not significant. The mating had significant effects on the expression of cry1 and cry2 mRNAs in H. armigera, and the expression levels of cry1 and cry2 mRNA in female and male decreased with the extension of time after mating. [Conclusion] The results are of great significance for the further study on the function of cry genes and the control of cotton bollworm.