Establishment of the Clone of Anemarrhena asphodeloides Rhizomes

• 作 者: GU Ting-ting LI Jie SONG Na XU Na JIANG Chang-yang
• 机 构: School of Life Science,Liaoning Normal University,
• 出 处: Medicinal Plant
• 发表日期: 2004年期
• 关键词: Clone germination planting differentiated protect inoculated medicinal meant clo

[Objective] To protect the wild resources of Anemarrhena asphodeloides and to meet the market demands for A.asphodeloides seedlings.[Method] With A.asphodeloides rhizomes as the explants,growth point germination,bud differentiation,tube seedlings rooting,and seedlings transplanting were conducted on based tissue culture technology.[Result] White+6-BA 0.1 mg/L+IAA 0.3 mg/L was the ideal culture medium for the growth point germination of A.asphodeloides rhizomes;MS+(1.0-1.5) mg/L 6-BA+0.2 mg/L NAA was the ideal culture medium for the differentiation of germinated buds;and 1/2 MS+0.1 mg/L NAA+(0.2-0.3) mg/L IAA was the ideal culture medium for the rooting of differentiated buds.[Conclusion] Asexual micro-propagation system of A.asphodeloides was successfully established.The transplanted tube seedlings had good growth vigor and were in the same growth speed.
[Objective] To protect the wild resources of Anemarrhena asphodeloides and to meet the market demands for A. asphodeloides seedlings. [Method] With A. asphodeloides rhizomes as the explants, growth point germination, bud differentiation, tube seedlings rooting, and seedlings transplanting were [Result] White + 6-BA 0.1 mg / L + IAA 0.3 mg / L was the ideal culture medium for the growth point of germination of A. asphodeloides rhizomes; MS + (1.0-1.5) mg / L 6-BA + 0.2 mg / L NAA was the ideal culture medium for the differentiation of germinated buds; and 1/2 MS + 0.1 mg / L NAA + (0.2-0.3) mg / L IAA was the ideal culture medium for the rooting of differentiated buds. [Conclusion] Asexual micro-propagation system of A. asphodeloides was successfully established. The transplanted tube seedlings had good growth vigor and were in the same growth speed.