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cDNA microarray is a powerful tool to analyze simultaneously the expression levels of tens ofthousands of genes.Compared with normal nasopharynx (NP) tissues,2210 genes were highly differentiallyexpressed in nasopharyngeal carcinoma (NPC) tissues detected by cDNA microarray.Since signal pathwayis widely used to describe the complex relationship between genes,a pathway-based network was con-structed to visualize the connection between the genes obtained from microarray data in this report.Weanalyzed the targeted genes that may have more important influence on this gene network with statisticalmethods and found that some genes might have significant influence on this network,especially Ras-relatednuclear protein (RAN),carboxyl ester lipase (CEL),v-rel reticuloendotheliosis viral oncogene homolog A(RELA) genes.To verify the results from pathway-based selection,reverse transcription-polymerase chainreaction (RT-PCR) and real-time RT-PCR were performed to detect the expression levels of RAN,CEL andRELA genes and it was found that the RAN and CEL genes were significantly up-regulated in more than 80%of NPC tissues.To further elucidate the function of the RAN gene,RAN expression was specifically sup-pressed in a 5-8F NPC cell line by RNA interference (RNAi).As expected,the depletion of RAN couldeffectively block the proliferation of tumor cells.Therefore,our study may open up a new way to analyze thevast microarray data.
cDNA microarray is a powerful tool to analyze simultaneously the expression levels of tens of thousands of of genes. Compared with normal nasopharynx (NP) tissues, 2210 genes were highly differentially expressed in nasopharyngeal carcinoma (NPC) tissues detected by cDNA microarray.Since signal pathway is widely used to describe the complex relationship between genes, a pathway-based network was con-structed to visualize the connection between the genes obtained from microarray data in this report.Weanalyzed the targeted genes that may have more important on this gene network with statistical methods and found that Some genes might have significant influence on this network, especially Ras-related nucleoprotein (RAN), carboxyl ester lipase (CEL), v-rel reticuloendotheliosis viral oncogene homolog A (RELA) genes.To verify the results from pathway-based selection, reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR were performed to detect the expression levels of RAN , CEL and RELA genes and it was found that the RAN and CEL genes were significantly up-regulated in more than 80% of NPC tissues. To further elucidate the function of the RAN gene, RAN expression was specifically sup-pressed in 5-8F NPC cell line by RNA interference (RNAi). As expected, the depletion of RAN couldeffectively block the proliferation of tumor cells. Before, our study may open up a new way to analyze thevast microarray data.