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目的 观察组胺对脑细胞内游离钙浓度的影响 ,并初步探讨其作用机制。方法 利用荧光探针Fura - 2测定乳鼠脑细胞内游离钙的浓度。结果 组胺可明显升高脑细胞内游离钙浓度 ,而异丙嗪可以阻断组胺的这一效应。结论 组胺升高脑细胞内游离钙浓度是由H1受体介导的。
Objective To investigate the effect of histamine on intracellular free calcium concentration in brain and its mechanism of action. Methods Fura - 2 fluorescent probe was used to determine the concentration of free calcium in neonatal rat brain cells. Results Histamine significantly increased intracellular free calcium concentration, while promethazine blocked this effect of histamine. Conclusions Histamine increases intracellular free calcium concentration mediated by H1 receptors.