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目的研究心肌型肌钙蛋白(cTnI)-mRNA在监测病毒性心肌损伤发生发展和预后中的价值。方法于BALB/c小鼠腹腔接种1×108TCID50柯萨奇病毒B3(CVB3)液诱发心肌损伤发生,分别在CVB3感染后第3、6、9、12、15、18和21天采集外周血后处死小鼠,留取鼠心脏作病理组织学检查,并以逆转录-聚合酶链反应(RT-PCR)分析心肌及外周血中cTnI基因的表达状态。结果CVB3感染后,鼠心肌组织及循环血中cTnI-mRNA均表达增加,且与心肌细胞肿胀、炎性细胞浸润、核固缩及碎裂、变性、坏死、钙化等组织学改变相关。cTnI-mRNA基因扩增,心肌组织全数阳性;外周血阳性率在对照组及感染后第3、6、9、12、15、18和21天分别为0、0、0、16.7%、40.0%、71.4%、83.3%和87.5%。结论病毒性心肌损伤时cTnI-mRNA上调表达并释放入血,循环血cTnI-mRNA为监测心肌损伤发生发展及预后的灵敏基因标志物。
Objective To investigate the value of cardiac troponin (cTnI) -mRNA in monitoring the development and prognosis of viral myocardium injury. Methods Myocardial injury was induced by intraperitoneal inoculation of 1 × 108 TCID50 Coxsackievirus B3 (CVB3) solution in BALB / c mice. Peripheral blood was collected on days 3, 6, 9, 12, 15, 18 and 21 after CVB3 infection The mice were sacrificed and the heart of the rats was removed for histopathological examination. The cTnI gene expression in myocardium and peripheral blood was analyzed by reverse transcription polymerase chain reaction (RT-PCR). Results After CVB3 infection, the expression of cTnI-mRNA in myocardium and circulating blood was increased, which was associated with histological changes such as myocardial cell swelling, inflammatory cell infiltration, nuclear pyknosis and fragmentation, degeneration, necrosis and calcification. cTnI-mRNA gene amplification, all the positive myocardial tissue; the positive rate of peripheral blood in the control group and infected on the 3rd, 6th, 9th, 12th, 15th, 18th and 21st days were 0,0,0,16.7%, 40.0% , 71.4%, 83.3% and 87.5% respectively. Conclusions cTnI-mRNA is up-regulated and released into the blood when viral myocardium is injured. Circulating blood cTnI-mRNA is a sensitive gene marker to monitor the development and prognosis of myocardial injury.