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目的研究ADM及其相关受体CRLR在正常、不孕妇女子宫内膜中的表达,探讨其与不孕的相关关系。方法收集14例原因不明不孕妇女与13例正常生育期妇女黄体中期(LH+6-LH+8)子宫内膜,通过免疫组化、半定量RT-PCR及实时荧光定量RT-PCR,比较两组内膜中ADM、CRLR蛋白表达和基因表达的差异。结果ADM蛋白表达出现在正常内膜的各个细胞中,而在不孕妇女内膜中间质细胞中表达低于正常对照组(P<0.05);CRLR蛋白表达则以血管内皮细胞表达为主,两组内膜各细胞中的表达无差异(P>0.05);不孕妇女内膜中血管密度值为4.81±0.25,明显低于正常妇女的7.83±0.79(P<0.05);ADM在基因水平的表达不孕组ADM mRNA表达3.99倍高于正常组,而CRLR mRNA表达两组无明显差异。结论ADM及其受体CRLR在正常子宫内膜的生成、修复中发挥重要作用;高表达的ADM mRNA尽管有助于种植窗口期孕卵的着床,但其对内膜血管的低调控可能是造成不孕的原因之一。
Objective To study the expression of ADM and its related receptor CRLR in normal and infertile women’s endometrium and to explore its relationship with infertility. Methods 14 cases of unexplained infertile women and 13 normal pregnant women were enrolled in the endometrial luteal phase (LH + 6-LH + 8). By immunohistochemistry, semi-quantitative RT-PCR and real-time quantitative RT-PCR Differences of ADM, CRLR protein expression and gene expression in the two groups of endometrium. Results The expression of ADM protein was found in normal endometrial cells, while it was lower in intima-media stromal cells of infertile women than in normal controls (P <0.05). The expression of CRLR protein was mainly expressed in vascular endothelial cells, There was no significant difference in the expression of endometrium between the two groups (P> 0.05). The intima-media thickness of infertile women was 4.81 ± 0.25, significantly lower than that of normal women (7.83 ± 0.79, P <0.05) The mRNA expression of ADM in infertility group was 3.99 times higher than that in normal group, while there was no significant difference in CRLR mRNA expression between the two groups. CONCLUSION ADM and its receptor CRLR play an important role in the formation and repair of normal endometrium. Although high expression of ADM mRNA may help implantation of ovum during the window period, its low regulation of endothelium may be Cause infertility one of the reasons.