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羟自由基(·OH)是一种活性氧自由基,具有细胞毒性,近年来有关其在生物学与医学上的作用进行了广泛的研究。在研究过程中,经常需要测知·OH的含量,关于·OH的测定方法有很多种,如ESR,HPLC,GLC及荧光法等。由于·OH是反应性很强的物种,存在寿命极短,故经常需加入各种捕获剂,荧光分析法即属于此种,在反应体系中,先加入一定量的苯甲酸,生成的·OH即刻被苯甲酸所捕获,生成
Hydroxyl radical (·OH) is a kind of active oxygen radical and has cytotoxicity. In recent years, it has been extensively studied regarding its role in biology and medicine. In the course of research, it is often necessary to measure the content of OH. There are many methods for measuring OH, such as ESR, HPLC, GLC, and fluorescence. Because OH is a very reactive species and has a very short life span, it is often necessary to add a variety of capture agents. Fluorescence analysis is such a method. In the reaction system, a certain amount of benzoic acid is added first, resulting in OH. Instantly captured by benzoic acid, generated