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菜豆普通花叶病毒(BCMV)、菜豆黄花叶病毒(BYMV)和南方菜豆花叶病毒(SBMV)是黑龙江省菜豆主要的种子传播病毒。为分析菜豆种质资源携带病毒的状况,以6份普通菜豆种子的胚根、胚芽和子叶为试材,采用CTAB法提取总RNA和基因组DNA,根据BCMV、BYMV和SBMV的衣壳蛋白基因序列设计特异引物,通过PCR和RT-PCR对180份菜豆资源进行病毒检测。结果表明:RT-PCR对BCMV、BYMV和SBMV的检出率分别为12.78%、9.44%和1.67%;PCR的检出率分别为12.78%、8.33%和1.67%。所收集的6种菜豆种质资源均检出1~3种病毒,其中BCMV和BYMV是主要的病毒种类。
Beet bean common mosaic virus (BCMV), bean yellow mosaic virus (BYMV) and southern bean mosaic virus (SBMV) are the major seed-transmitted viruses of kidney bean in Heilongjiang Province. In order to analyze the virus carrying status of kidney bean germplasm resources, the total RNA and genomic DNA were extracted from 6 radicles, germ and cotyledon of common bean seeds using CTAB method. Based on the BCMV, BYMV and SBMV capsid protein gene sequences Specific primers were designed to detect the virus in 180 Beans lines by PCR and RT-PCR. The results showed that the detection rates of BCMV, BYMV and SBMV by RT-PCR were 12.78%, 9.44% and 1.67%, respectively. The detection rates of PCR were 12.78%, 8.33% and 1.67% respectively. The collected 6 kinds of kidney bean germplasm resources were detected 1 to 3 kinds of viruses, including BCMV and BYMV is the main virus species.