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目的:探讨CDCA8和INCENP mRNA在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及其临床意义。方法:使用Illumina Nextbio芯片数据库分析原发性HCC组织和相应癌旁组织中CDCA8和INCENP mRNA表达情况,在癌症基因组图谱(The Cancer Genome Atlas,TCGA)肝癌数据库中使用RNA-Seq mRNA表达数据进行验证,并结合TCGA临床数据分析CDCA8和INCENP mRNA表达水平与HCC患者临床病理特征及预后的关系,使用基因集富集软件分析目的基因高表达的相关通路。结果:CDCA8和INCENP mRNA在HCC组织中表达水平显著上调(P<0.01)。CDCA8 mRNA表达水平与HCC的组织学分级、瘤体大小、肿瘤复发、美国东部肿瘤协作组织(ECOG)评级、血清AFP水平、肿瘤基因拷贝数变异显著相关(P<0.05);INCENP mRNA表达水平与HCC的组织学分级、肿瘤复发、ECOG评级、血清AFP水平、肿瘤基因突变总数和肿瘤基因拷贝数变异显著相关(P<0.05)。CDCA8高表达组患者的OS及TFS显著低于CDCA8低表达组患者(P<0.01);INCENP高表达组患者的OS显著低于INCENP低表达组患者(P<0.05),而TFS无显著差异(P>0.05)。多因素分析显示,CDCA8 mRNA水平是预测HCC患者不良预后的独立因素(P<0.01)。基因集富集分析表明,CDCA8和INCENP可能在细胞周期之外的其他生物学信号通路中发挥一定作用。结论:CDCA8和INCENP mRNA在HCC组织中呈现显著高表达。CDCA8可能成为HCC预后的独立风险因素和新的治疗靶点。
Objective: To investigate the expression of CDCA8 and INCENP mRNA in hepatocellular carcinoma (HCC) tissues and their clinical significance. METHODS: CDCA8 and INCENP mRNA expression in primary HCC tissues and corresponding paracancerous tissues was analyzed using the Illumina Nextbio chip database and verified using RNA-Seq mRNA expression data in the Cancer Genome Atlas (TCGA) liver cancer database , And combined with TCGA clinical data analysis of CDCA8 and INCENP mRNA expression in HCC patients with clinicopathological features and prognosis, the use of gene set enrichment software analysis of the target gene overexpression related pathways. Results: The expressions of CDCA8 and INCENP mRNA in HCC tissues were significantly up-regulated (P <0.01). The expression level of CDCA8 mRNA was significantly correlated with histological grade, tumor size, tumor recurrence, ECOG grade, serum AFP level and gene copy number variation (P <0.05). The expression of INCENP mRNA was correlated with HCC histological grade, tumor recurrence, ECOG grade, serum AFP level, the total number of gene mutations and tumor gene copy number variation was significantly (P <0.05). The OS and TFS of CDCA8 overexpression patients were significantly lower than those of CDCA8 low expression patients (P <0.01). The OS of patients with high expression of INCENP was significantly lower than that of patients with low INCENP expression (P <0.05), but no significant difference of TFS P> 0.05). Multivariate analysis showed that CDCA8 mRNA level was an independent predictor of poor prognosis in patients with HCC (P <0.01). Gene enrichment analysis showed that CDCA8 and INCENP may play a role in other biological signaling pathways beyond the cell cycle. Conclusion: CDCA8 and INCENP mRNA are highly expressed in HCC tissues. CDCA8 may become an independent risk factor for HCC prognosis and a new therapeutic target.